Human S100 Calcium Binding Protein A12 (S100A12) RTU ELISA Kit | S100A12 rtu elisa kit

Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit

Cat. Num. AAA4502313

• Gene Names: S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein A12 (S100A12); Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit; CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6; S100A12 rtu elisa kit

• Reactivity: Human
• Sequence Length: 92

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 34pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A12 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to S100A12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100A12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5032059
• NCBI GeneID: 6283
• NCBI Accession #: NP_005612.1
• NCBI GenBank Nucleotide #: NM_005621.1
• UniProt Accession #: P80511; P83219; Q5SY66; Q7M4R1
• Molecular Weight: 10,575 Da
• NCBI Official Full Name: protein S100-A12
• NCBI Official Synonym Full Names: S100 calcium binding protein A12
• NCBI Official Symbol: S100A12
• NCBI Official Synonym Symbols: p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
• NCBI Protein Information: protein S100-A12
• UniProt Protein Name: Protein S100-A12
• Protein Family: Protein
• UniProt Gene Name: S100A12
• UniProt Synonym Gene Names: CAAF1; CAGC; EN-RAGE; MRP-6; p6

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]

Uniprot Description

S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn2+ from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.