Rubella Virus Antigens Antigen
Rubella Grade IV Antigen (HPV77)
Applications
ELISA, Western Blot
Synonyms
Rubella Virus Antigens; Rubella Grade IV Antigen (HPV77); Rubella Grade IV Antigen (Strain HPV77) >90% Viral Protein; PBS; Rubella Virus Antigens antigen
Host
Grown in Vero Cells.
Form/Format
Purified Liquid
Applicable Applications for Rubella Virus Antigens antigen
ELISA, Western Blot
Application Notes
Optimal dilutions/concentrations should be determined by the end user.
Host Note
Product manufactured in the United States using only domestic animal materials processed in U.S. facilities.
Protein Concentration
BCA Assay:
Acceptable Range: ≥ 0.250 mg/mL
Testing Results: 0.350 mg/mL
OD 260/280mn Assay: Acceptable Range: 045 - 0.60 mg/mL
Testing Results: 0.54 mg/mL
Acceptable Range: ≥ 0.250 mg/mL
Testing Results: 0.350 mg/mL
OD 260/280mn Assay: Acceptable Range: 045 - 0.60 mg/mL
Testing Results: 0.54 mg/mL
Preservative
None
Activity
Testing Results: Midpoint Titer (test antigen): 332.562
ELISA Assay: Acceptable Range: -15% - +50% of Reference Antigen % of Reference: 3.86%
Testing Results: Midpoint Titer (Reference Antigen): 320.211
Background ELISA Ratio: Acceptable ≤ 2; Test Result: 1
Sensitivity/Specificity Negative Control: Acceptable to Report; Test Result: Acceptable
Sensitivity/Specificity Medium Positive Control: Acceptable ≥ 3; Test Result: 15
Sensitivity/Specificity High Positive Control: Acceptable ≥ 8; Test Result: 31
ELISA Assay: Acceptable Range: -15% - +50% of Reference Antigen % of Reference: 3.86%
Testing Results: Midpoint Titer (Reference Antigen): 320.211
Background ELISA Ratio: Acceptable ≤ 2; Test Result: 1
Sensitivity/Specificity Negative Control: Acceptable to Report; Test Result: Acceptable
Sensitivity/Specificity Medium Positive Control: Acceptable ≥ 3; Test Result: 15
Sensitivity/Specificity High Positive Control: Acceptable ≥ 8; Test Result: 31
Inactivation
Product inactivated by UV irradiation and verified by Infectivity Testing (Plaque Assay)
Acceptable Range: ≤ 10 PFU/mL
Testing Results: 0 PFU/mL
Acceptable Range: ≤ 10 PFU/mL
Testing Results: 0 PFU/mL
Usage Notes
Recommend sonication using microprobe set to 10% amplitude. Apply I - 3 second pulses with sample on ice.
Buffer
PBS with Sucrose, without Sodium azide, pH 7.4 - 7.7
Preparation and Storage
Store at ≤ -65°C.
Avoid multiple freeze/thaw cycles.
Avoid multiple freeze/thaw cycles.
Related Product Information for Rubella Virus Antigens antigen
Rubella Virus Grade IV PBS antigen.
Introduction: Rubella (German Measles) Virus Antigen is medically significant because of its teratogenic effect when contracted by childbearing women. Maternal infection, especially during the first trimester of pregnancy, can result in a range of congenital birth defects including deafness, cataracts, diabetes and cardiac and bone abnormalities. The characteristic multi-system defects resulting from a Rubella prenatal infection are termed the Congenital Rubella Syndrome (CRS).
Serologic tests are an important aid in the prevention of prenatal Rubella infections. The incidence of this disease has decreased dramatically in the United States following the nationwide Rubella testing and immunization program.
Rubella Virus Antigen is prepared by the concentration of cell culture fluid from cells infected with Rubella Virus strain HPV77. The Rubella virus is purified by gradient centrifugation and yields an antigen high in sensitivity with low backgrounds in the ELISA and other solid phase immunoassays. The Rubella Grade IV product #6076 and #6123 are ~90% pure and are typically required for IgM antibody testing. All of the Rubella products are inactivated and are tested for infectivity prior to release.
Introduction: Rubella (German Measles) Virus Antigen is medically significant because of its teratogenic effect when contracted by childbearing women. Maternal infection, especially during the first trimester of pregnancy, can result in a range of congenital birth defects including deafness, cataracts, diabetes and cardiac and bone abnormalities. The characteristic multi-system defects resulting from a Rubella prenatal infection are termed the Congenital Rubella Syndrome (CRS).
Serologic tests are an important aid in the prevention of prenatal Rubella infections. The incidence of this disease has decreased dramatically in the United States following the nationwide Rubella testing and immunization program.
Rubella Virus Antigen is prepared by the concentration of cell culture fluid from cells infected with Rubella Virus strain HPV77. The Rubella virus is purified by gradient centrifugation and yields an antigen high in sensitivity with low backgrounds in the ELISA and other solid phase immunoassays. The Rubella Grade IV product #6076 and #6123 are ~90% pure and are typically required for IgM antibody testing. All of the Rubella products are inactivated and are tested for infectivity prior to release.
Product Categories/Family for Rubella Virus Antigens antigen
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Product Notes
The Rubella Virus Antigens (Catalog #AAA318672) is an Antigen produced from Grown in Vero Cells. and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rubella Virus Antigens can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the Rubella Virus Antigens for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rubella Virus Antigens, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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