GroES Recombinant Protein | groS recombinant protein
GroES, Recombinant (Chaperonin 10, cpn10, Heat Shock Protein 10, HSP10)
Purity
Highly Purified95% 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Coomassie.
Synonyms
GroES; Recombinant (Chaperonin 10; cpn10; Heat Shock Protein 10; HSP10); groS recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
95% 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Coomassie.
95% 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Coomassie.
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 5mM DTT and 10% glycerol.
Sequence
MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 0.1% HSA or BSA, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
Related Product Information for groS recombinant protein
GroES protein is the co-chaperonin of GroES in E. coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kD subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E. coli and cloned into an expression vector. This protein was overexpressed in E. coli and was purified by using conventional chromatography techniques.
Recombinant GroES produced in E. coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 10.4kD.
Recombinant GroES produced in E. coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 10.4kD.
Product Categories/Family for groS recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
10.4kD
NCBI Official Full Name
GroES
UniProt Protein Name
10 kDa chaperonin
Protein Family
UniProt Gene Name
groS
UniProt Synonym Gene Names
groES
UniProt Entry Name
CH10_STRCO
Uniprot Description
Function: Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. HAMAP MF_00580
Subunit structure: Heptamer of 7 subunits arranged in a ring
Subcellular location: Cytoplasm
By similarity HAMAP MF_00580.
Sequence similarities: Belongs to the GroES chaperonin family.
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Product Notes
The GroES gros (Catalog #AAA650271) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA. It is sometimes possible for the material contained within the vial of "GroES, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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