Glutamate Receptor Recombinant Protein | GRIA2 recombinant protein

Recombinant Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)

Cat. Num. AAA2029706

• Gene Names: GRIK1; EAA3; EEA3; GLR5; GLUR5; GluK1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Glutamate Receptor; Recombinant Glutamate Receptor; Ionotropic; AMPA 2 (GRIA2); GRIA2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; NSIQIG GLFPRGADQE YSAFRVGMVQ FSTSEFRLTP HIDNLEVANS FAVTNAFCSQ FSRGVYAIFG FYDKKSVNTI TSFCGTLHVS FITPSFPTDG THPFVIQMRP DLKGALLSLI EYYQWDKFAY LYDSDRGLST LQAVLDSAAE KKWQVTAINV GNINNDKKDE MYRSLFQDLE LKKERRVILD CERDKVNDIV DQVITIGKHV KGYHYIIANL GFTDGDLLKI QFGGANVSGF QIVDYDDSLV SKFIERWSTL EEKEYPGAHT TTIKYTSALT YDAVQVMTEA FRNLRKQRIE ISRRGNAGDC LANPAVPWGQ GVEIERALKQ VQVEGLSGNI KFDQNGKRIN YTINIMELKT NGPRKIGYWS EVDKMVVTLT ELPSGNDTSG LENKTVVVTT ILESPYVMMK KNHEMLEGNE RYEGYCVDLA AEIAKHCGFK YKLTIVGDGK YGARDADTKI WNGMVGELVY GKADIAIAPLTITLVREEVI DFSKPFMSLG ISIMIKKPQK SKPGVFSFLD PLA
• Sequence Length: 51

• Applicable Applications for GRIA2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 62.0KDa
• Accurate Molecular Mass (KD): 62kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Asn25~Ala543
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4154321
• NCBI GeneID: 2897
• Molecular Weight: 102,631 Da
• NCBI Official Full Name: glutamate receptor, partial
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, kainate 1
• NCBI Official Symbol: GRIK1
• NCBI Official Synonym Symbols: EAA3; EEA3; GLR5; GLUR5; GluK1
• NCBI Protein Information: glutamate receptor ionotropic, kainate 1
• UniProt Protein Name: Glutamate receptor ionotropic, kainate 1
• Protein Family: Glutamate receptor
• UniProt Gene Name: GRIK1
• UniProt Synonym Gene Names: GLUR5; GluK1; EAA3; GluR-5; GluR5
• UniProt Entry Name: GRIK1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR5: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: presynaptic membrane; postsynaptic membrane; kainate selective glutamate receptor complex; integral to plasma membrane; dendrite; plasma membrane; cell junction

Molecular Function: kainate selective glutamate receptor activity; extracellular-glutamate-gated ion channel activity

Biological Process: nervous system development; synaptic transmission, glutamatergic; central nervous system development; regulation of synaptic transmission, glutamatergic; glutamate signaling pathway; positive regulation of synaptic transmission, GABAergic; negative regulation of synaptic transmission, glutamatergic; regulation of inhibitory postsynaptic membrane potential; synaptic transmission; behavioral response to pain; ionotropic glutamate receptor signaling pathway; transport; adult behavior; regulation of excitatory postsynaptic membrane potential; positive regulation of gamma-aminobutyric acid secretion