Fetuin-A / AHSG / FETUA Recombinant Protein | AHSG / FETUA recombinant protein

Recombinant Human Fetuin-A / AHSG / FETUA Protein (His tag)

Cat. Num. AAA2546342

• Gene Names: AHSG; AHS; A2HS; HSGA; FETUA
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Fetuin-A / AHSG / FETUA; Recombinant Human Fetuin-A / AHSG / FETUA Protein (His tag); Ahsg; Feetuin A; AHSG / FETUA recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.2
• Sequence Length: 367

• Application Notes: The secreted recombinant human Fetuin A consists of 360 amino acids and predicts a molecular mass of 38.8 kDa. By SDS-PAGE under reducing conditions, rhFetuinA migrates as an approximately 55-60 kDa protein due to glycosylation.
• Predicted N Terminal: Ala 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for AHSG / FETUA recombinant protein

Background: Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.

Description: A DNA sequence encoding the human Fetuin A (NP_001613.2) (Met 1-Val 367) precursor was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 156523970
• NCBI GeneID: 197
• NCBI Accession #: NP_001613.2
• NCBI GenBank Nucleotide #: NM_001622.2
• UniProt Accession #: P02765; O14961; O14962; Q9P152; A8K9N6; B2R7G1; AAB29984
• Molecular Weight: 39,325 Da
• NCBI Official Full Name: alpha-2-HS-glycoprotein preproprotein
• NCBI Official Synonym Full Names: alpha 2-HS glycoprotein
• NCBI Official Symbol: AHSG
• NCBI Official Synonym Symbols: AHS; A2HS; HSGA; FETUA
• NCBI Protein Information: alpha-2-HS-glycoprotein
• UniProt Protein Name: Alpha-2-HS-glycoprotein
• UniProt Gene Name: AHSG
• UniProt Synonym Gene Names: FETUA

NCBI Description

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]

Uniprot Description

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27.3

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: endopeptidase inhibitor activity

Biological Process: acute-phase response; negative regulation of bone mineralization; platelet degranulation; positive regulation of phagocytosis; regulation of inflammatory response