GREMLIN Blocking Peptide | Grem1 blocking peptide

GREMLIN Antibody (C-term) Blocking Peptide

• Gene Names: Grem1; ld; Drm; Grem; Cktsf1b1
• Synonyms: GREMLIN; GREMLIN Antibody (C-term) Blocking Peptide; Gremlin-1; Cysteine knot superfamily 1; BMP antagonist 1; Down-regulated in Mos-transformed cells protein; Grem1; Cktsf1b1; Drm; Grem1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human GREMLIN. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 184

• Cellular Location: Secreted.
• Tissue Location: Highly expressed in spleen and to a lesser extent in lung, skeletal muscle and kidney. Expressed only in non- transformed cells or primary fibroblasts in culture but not in established transformed or tumor derived cell lines. Broadly expressed in limb bud mesenchyme but restricted to the distal limb bud mesenchyme and concentrated posteriorly. Expressed in ovary especially in granulosa cells of follicles of type 4
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Grem1 blocking peptide

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis.

NCBI and Uniprot Product Information

• NCBI GI #: 62510669
• NCBI GeneID: 23892
• UniProt Accession #: O70326
• Molecular Weight: 20,710 Da
• NCBI Official Full Name: Gremlin-1
• NCBI Official Synonym Full Names: gremlin 1, DAN family BMP antagonist
• NCBI Official Symbol: Grem1
• NCBI Official Synonym Symbols: ld; Drm; Grem; Cktsf1b1
• NCBI Protein Information: gremlin-1
• UniProt Protein Name: Gremlin-1
• Protein Family: Gremlin
• UniProt Gene Name: Grem1
• UniProt Synonym Gene Names: Cktsf1b1; Drm
• UniProt Entry Name: GREM1_MOUSE

Uniprot Description

GREM1: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. Interacts with SLIT1 and SLIT2 in a glycosylation- dependent manner. By high glucose through TGFB1-mediated pathways in mesangial cell. Down-regulated in tumor cell lines. Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Belongs to the DAN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: cell surface; extracellular space

Molecular Function: heparan sulfate proteoglycan binding; receptor agonist activity; transmembrane receptor protein tyrosine kinase activator activity; vascular endothelial growth factor receptor 2 binding

Biological Process: activation of NF-kappaB transcription factor; angiogenesis; cell migration during sprouting angiogenesis; cell morphogenesis; cell-cell signaling; collagen fibril organization; determination of dorsal identity; embryonic limb morphogenesis; endothelial cell migration; limb development; negative regulation of apoptosis; negative regulation of BMP signaling pathway; negative regulation of bone mineralization; negative regulation of bone remodeling; negative regulation of cell growth; negative regulation of chondrocyte differentiation; negative regulation of leukocyte chemotaxis; negative regulation of osteoblast proliferation; negative regulation of transcription, DNA-dependent; organ morphogenesis; positive regulation of angiogenesis; positive regulation of cell proliferation; positive regulation of NF-kappaB import into nucleus; positive regulation of receptor internalization; positive regulation of telomerase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proximal/distal pattern formation; regulation of focal adhesion formation; regulation of stress-activated MAPK cascade; signal transduction; ureteric bud branching