Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded OVCAR3 xenograft using Alkaline Phosphatase antibody at a dilution of 1:100)
Rabbit Alkaline Phosphatase Polyclonal Antibody | anti-ALP antibody
Alkaline Phosphatase Antibody
Applications
Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
Alkaline Phosphatase; Polyclonal Antibody; Alkaline Phosphatase Antibody; Affinity Purified Rabbit Polyclonal Alkaline Phosphatase Antibody; Polyclonal Alkaline Phosphatase antibody; Anti-Alkaline Phosphatase antibody; ALPL antibody; alkaline phosphatase tissue-nonspecific isozyme antibody; HOPS antibody; TNAP antibody; TNSALP antibody; MGC161443 antibody; MGC167935 antibody; FLJ93059 antibody; glycerophosphatase antibody; FLJ40094 antibody; liver/bone/kidney-type alkaline phosphatase antibody; alkaline phosphatase liver/bone/kidney isozyme antibody; APTNAP antibody; tissue-nonspecific ALP antibody; AP-TNAP antibody; alkaline phosphomonoesterase antibody; anti-ALP antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.48 mg/ml (varies by lot)
Sequence Length
368
Applicable Applications for anti-ALP antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:500
WB: 1:500-1:3000
WB: 1:500-1:3000
Immunogen
Alkaline Phosphatase antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 359 of Alkaline Phosphatase
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded OVCAR3 xenograft using Alkaline Phosphatase antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded OVCAR3 xenograft using Alkaline Phosphatase antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5% SDS PAGE gel and Alkaline Phosphatase antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5% SDS PAGE gel and Alkaline Phosphatase antibody at a dilution of 1:1000)
Related Product Information for anti-ALP antibody
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.
Product Categories/Family for anti-ALP antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
57 kDa
NCBI Official Full Name
Alkaline phosphatase
Protein Family
Similar Products
Product Notes
The ALP (Catalog #AAA5311788) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alkaline Phosphatase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the ALP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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