Rat G protein-coupled receptor 94 ELISA Kit | GPR94 elisa kit

Rat G protein-coupled receptor 94 ELISA Kit

Cat. Num. AAA750814

• Gene Names: P2RY13; GPCR1; GPR86; GPR94; P2Y13; SP174; FKSG77
• Reactivity: Rat
• Synonyms: G protein-coupled receptor 94; Rat G protein-coupled receptor 94 ELISA Kit; GPR94 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This GPR94 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GPR94. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This GPR94 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GPR94. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPR94 elisa kit

Principle of the Assay: GPR94 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPR94 antibody and an GPR94-HRP conjugate. The assay sample and buffer are incubated together with GPR94-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPR94 concentration since GPR94 from samples and GPR94-HRP conjugate compete for the anti-GPR94 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPR94 from the sample, fewer sites are left to bind GPR94-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPR94 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GPR94 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 194294562
• NCBI GeneID: 53829
• NCBI Accession #: NP_795713.2
• NCBI GenBank Nucleotide #: NM_176894.2
• Molecular Weight: 38,410 Da
• NCBI Official Full Name: P2Y purinoceptor 13
• NCBI Official Synonym Full Names: purinergic receptor P2Y, G-protein coupled, 13
• NCBI Official Symbol: P2RY13
• NCBI Official Synonym Symbols: GPCR1; GPR86; GPR94; P2Y13; SP174; FKSG77
• NCBI Protein Information: P2Y purinoceptor 13; G protein-coupled receptor 86; G-protein coupled receptor 86; G-protein coupled receptor 94
• UniProt Protein Name: P2Y purinoceptor 13
• UniProt Gene Name: P2RY13
• UniProt Synonym Gene Names: GPR86; GPR94; FKSG77; P2Y13
• UniProt Entry Name: P2Y13_HUMAN

NCBI Description

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is activated by ADP. [provided by RefSeq, Sep 2008]

Uniprot Description

P2RY13: Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: integral to plasma membrane; endoplasmic reticulum; plasma membrane

Molecular Function: purinergic nucleotide receptor activity, G-protein coupled

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of adenylate cyclase activity