GPNMB recombinant protein

GPNMB, 22-474aa, Human, His-tag, Baculovirus

Cat. Num. AAA206529

• Gene Names: GPNMB; NMB; HGFIN
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GPNMB; 22-474aa; Human; His-tag; Baculovirus; Glycoprotein nmb; NMB; HGFIN; GPNMB recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by BCA assay) (varies by lot)
• Sequence: ADPAKRFHDV LGNERPSAYM REHNQLNGWS SDENDWNEKL YPVWKRGDMR WKNSWKGGRV QAVLTSDSPA LVGSNITFAV NLIFPRCQKE DANGNIVYEK NCRNEAGLSA DPYVYNWTAW SEDSDGENGT GQSHHNVFPD GKPFPHHPGW RRWNFIYVFH TLGQYFQKLG RCSVRVSVNT ANVTLGPQLM EVTVYRRHGR AYVPIAQVKD VYVVTDQIPV FVTMFQKNDR NSSDETFLKD LPIMFDVLIH DPSHFLNYST INYKWSFGDN TGLFVSTNHT VNHTYVLNGT FSLNLTVKAA APGPCPPPPP PPRPSKPTPS LGPAGDNPLE LSRIPDENCQ INRYGHFQAT ITIVEGILEV NIIQMTDVLM PVPWPESSLI DFVVTCQGSI PTEVCTIISD PTCEITQNTV CSPVDVDEMC LLTVRRTFNG SGTYCVNLTL GDDTSLALTS TLISVPHHHH HH
• Sequence Length: 560

• Applicable Applications for GPNMB recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GPNMB recombinant protein

GPNMB, also known as glycoprotein nmb, is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. This protein participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages. It may be involved in growth delay and reduction of metastatic potential. Recombinant human GPNMB protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for GPNMB recombinant protein

Immunology

References

Huang JJ., et al. (2012) Brain Behav. 2: 85-96.; Kumagai K., et al. (2015) PLoS One. 10: e0143413.

NCBI and Uniprot Product Information

• NCBI GI #: 4505405
• NCBI GeneID: 10457
• NCBI Accession #: NP_002501
• NCBI GenBank Nucleotide #: NM_002510.2
• UniProt Accession #: Q14956; Q6UVX1; Q8N1A1; A4D155
• Molecular Weight: 51.8kDa (462aa) 50-70KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: transmembrane glycoprotein NMB isoform b
• NCBI Official Synonym Full Names: glycoprotein nmb
• NCBI Official Symbol: GPNMB
• NCBI Official Synonym Symbols: NMB; HGFIN
• NCBI Protein Information: transmembrane glycoprotein NMB
• UniProt Protein Name: Transmembrane glycoprotein NMB
• Protein Family: Transmembrane glycoprotein
• UniProt Gene Name: GPNMB
• UniProt Synonym Gene Names: HGFIN; NMB

NCBI Description

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Could be a melanogenic enzyme.