Human Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 ELISA Kit | GPIHBP1 elisa kit

Human Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPIHBP1) ELISA Kit

Cat. Num. AAA282857

• Gene Names: GPIHBP1; HYPL1D; GPI-HBP1
• Reactivity: Human
• Synonyms: Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1; Human Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPIHBP1) ELISA Kit; GPIHBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human AGL. No significant cross-reactivity or interference between Human AGL and analogues was observed.
• Sequence Length: 184

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/mL-2000 pg/mL
• Sensitivity: 15.6 pg/mL (mean of 6 independent assays).
• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GPIHBP1 elisa kit

Intended Uses: For the quantitative detection of Human Glycogen debranching enzyme (AGL) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate AGL in Human serum, plasma. An antibody specific for AGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 684179339
• NCBI GeneID: 338328
• NCBI Accession #: NP_001288701.1
• NCBI GenBank Nucleotide #: NM_001301772.1
• UniProt Accession #: Q8IV16; Q6P3T2; Q86W15
• Molecular Weight: 19,806 Da
• NCBI Official Full Name: glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1
• NCBI Official Symbol: GPIHBP1
• NCBI Official Synonym Symbols: HYPL1D; GPI-HBP1
• NCBI Protein Information: glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
• UniProt Protein Name: Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1
• Protein Family: Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein
• UniProt Gene Name: GPIHBP1
• UniProt Synonym Gene Names: HBP1; GPI-HBP1; GPI-anchored HDL-binding protein 1

NCBI Description

This gene encodes a capillary endothelial cell protein that facilitates the lipolytic processing of triglyceride-rich lipoproteins. The encoded protein is a glycosylphosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly6) family. This protein plays a major role in transporting lipoprotein lipase (LPL) from the subendothelial spaces to the capillary lumen. Mutations in this gene are the cause of hyperlipoproteinemia, type 1D. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

GPIHBP1: Plays a key role in the lipolytic processing of chylomicrons.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: anchored component of external side of plasma membrane; apical plasma membrane; basolateral plasma membrane; external side of plasma membrane; extracellular region; intracellular; plasma membrane

Molecular Function: high-density lipoprotein particle binding; lipid binding; protein transmembrane transporter activity

Biological Process: C-terminal protein lipidation; cholesterol homeostasis; intracellular protein transport; lipid transport; positive regulation of lipoprotein lipase activity; protein import; protein stabilization; regulation of lipoprotein lipase activity; retinoid metabolic process; transcytosis

Disease: Hyperlipoproteinemia, Type Id