Typical Testing Data/Standard Curve (for reference only)
Rat glypican 3 ELISA Kit | GPC3 elisa kit
Rat Glypican-3, GPC3 ELISA Kit
Gene Names
Gpc3; OCI-5
Reactivity
Rat
Synonyms
glypican 3; Rat Glypican-3; GPC3 ELISA Kit; Rat Glypican-3 (GPC3) ELISA kit; AC002420.1; DGSX; GTR2-2; MXR7; OCI-5; SDYS; SGB; SGBS; SGBS1; glypican proteoglycan 3; heparan sulphate proteoglycan; intestinal protein OCI-5; secreted glypican-3; GPC3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat GPC3. No significant cross-reactivity or interference between Rat GPC3 and analogues was observed.
Sequence Length
597
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GPC3 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPC3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,049 Da
NCBI Official Full Name
glypican-3
NCBI Official Synonym Full Names
glypican 3
NCBI Official Symbol
Gpc3
NCBI Official Synonym Symbols
OCI-5
NCBI Protein Information
glypican-3; proteoglycan GPC3; intestinal protein OCI-5; defective in Simpson-Golabi-Behmel overgrowth syndrome
UniProt Protein Name
Glypican-3
Protein Family
UniProt Gene Name
Gpc3
UniProt Entry Name
GPC3_RAT
NCBI Description
heparan sulfate cell surface proteogycan, may be involved in development of mesodermal tissues, may modulate Igf2 function [RGD, Feb 2006]
Uniprot Description
Function: Inhibits the dipeptidyl peptidase activity of DPP4 Cell surface proteoglycan that bears heparan sulfate. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Research Articles on GPC3
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Product Notes
The Rat GPC3 gpc3 (Catalog #AAA926098) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA926098 ELISA Kit recognizes Rat GPC3. It is sometimes possible for the material contained within the vial of "glypican 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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