SDS-Page
(SDS-PAGE: Purified recombinant HIV-1 gp140 (Clade B) protein)
gp140 (HIV-1/Clade B) Recombinant Protein | gp140 recombinant protein
gp140 (HIV-1/Clade B), Timer, HEK293
Applications
Western Blot, ELISA
Purity
>= 95% purity (SDS PAGE)
Synonyms
gp140 (HIV-1/Clade B); Timer; HEK293; with a Trimer Domain; gp140 recombinant protein
Host
Glycosylated recombinant HIV-1 gp140 protein purified from 293 cells
Purity/Purification
>= 95% purity (SDS PAGE)
Concentration
1mg/ml in PBS (varies by lot)
Applicable Applications for gp140 recombinant protein
Western Blot (WB) standard, antibody ELISA (EIA), HIV-1 entry inhibition, Immunogen
Endotoxin
<0.1 EU per 1ug of the protein by LAL test
Preparation and Storage
Store at -20 degree C. Stable for 6-months from the date of shipment when kept at 4 degree C. Non hazardous.
SDS-Page
(SDS-PAGE: Purified recombinant HIV-1 gp140 (Clade B) protein)
SDS-Page
(SDS-PAGE: Purified recombinant HIV-1 gp140 (Clade B) protein)
Related Product Information for gp140 recombinant protein
Introduction: The exposed trimeric envelope glycoproteins of HIV target viral entry by melding the membranesof host cells with the virus. It consists of a structure of heterodimers of the transmembraneglycoprotein (gp41) and a surface glycoprotein (gp120) which are both derived by proteolyticcleavage from their common gp160 precursor. The gp140 ectodomain of envelope glycoprotein isa heterodimer with mass of approximately 140 kDa, composed of the entire gp120 component,and approximately 20 kDa of gp41. This ectodomain is essential for viral entry into cells as itplays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN,Heparan Sulfate Proteoglycan and a specific interaction with the CD4 receptor, particularly onhelper T-cells. Binding to CD4 induces the start of a cascade of conformational changes inGP120 and gp41 that lead to the fusion of the viral with the host cell membrane. Binding to CD4is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.
Description: Recombinant gp140 protein expressed and purified from HEK293 cells. Full length HIV-1 gp140 (Clade B) protein (amino acid 23-680) with a C-terminal foldon-6xhis tag (GenBank Accession No. M63929); A linker sequence (SGGGGSGGGGSGGGG) has been introduced between gp120 and gp41. Majority of protein exists as trimer.
Description: Recombinant gp140 protein expressed and purified from HEK293 cells. Full length HIV-1 gp140 (Clade B) protein (amino acid 23-680) with a C-terminal foldon-6xhis tag (GenBank Accession No. M63929); A linker sequence (SGGGGSGGGGSGGGG) has been introduced between gp120 and gp41. Majority of protein exists as trimer.
Product Categories/Family for gp140 recombinant protein
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Product Notes
The gp140 (Catalog #AAA430609) is a Recombinant Protein produced from Glycosylated recombinant HIV-1 gp140 protein purified from 293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's gp140 (HIV-1/Clade B) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) standard, antibody ELISA (EIA), HIV-1 entry inhibition, Immunogen. Researchers should empirically determine the suitability of the gp140 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "gp140 (HIV-1/Clade B), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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