Sheep Anti-glycoprotein antibody ELISA Kit | GP elisa kit

Sheep Anti-glycoprotein antibody ELISA Kit

Cat. Num. AAA750516

• Reactivity: Sheep
• Synonyms: Anti-glycoprotein antibody; Sheep Anti-glycoprotein antibody ELISA Kit; GP elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This GP-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep GP-Ab. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This GP-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep GP-Ab. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GP elisa kit

Principle of the Assay: GP-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing glycoprotein antigen and an GP-Ab-HRP conjugate. The assay sample and buffer are incubated together with GP-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GP-Ab concentration since GP-Ab from samples and GP-Ab-HRP conjugate compete for the glycoprotein antigen binding site. Since the number of sites is limited, as more sites are occupied by GP-Ab from the sample, fewer sites are left to bind GP-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GP-Ab concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 348912
• Molecular Weight: 42,625 Da
• NCBI Official Full Name: glycoprotein
• UniProt Protein Name: Chitinase-3-like protein 1
• Protein Family: Envelopment polyprotein
• UniProt Gene Name: CHI3L1
• UniProt Synonym Gene Names: CGP-39; GP-39; hCGP-39
• UniProt Entry Name: CH3L1_HUMAN

Uniprot Description

CHI3L1: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7). Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. Belongs to the glycosyl hydrolase 18 family.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm

Molecular Function: extracellular matrix structural constituent; chitin binding; chitinase activity; carbohydrate binding

Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; response to mechanical stimulus; apoptosis; cartilage development; carbohydrate metabolic process; activation of NF-kappaB-inducing kinase; immune response; chitin catabolic process; inflammatory response; lung development

Disease: Schizophrenia; Asthma-related Traits, Susceptibility To, 7