Rabbit Cartilage glycoprotein 39 ELISA Kit | gp-39 elisa kit

Rabbit Cartilage glycoprotein 39 ELISA Kit

Cat. Num. AAA720518

• Gene Names: CHI3L1; GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
• Reactivity: Rabbit
• Synonyms: Cartilage glycoprotein 39; Rabbit Cartilage glycoprotein 39 ELISA Kit; gp-39 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This gp-39 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit gp-39. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for gp-39 elisa kit

Principle of the assay: gp-39 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gp-39 antibody and an gp-39-HRP conjugate. The assay sample and buffer are incubated together with gp-39-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gp-39 concentration since gp-39 from samples and gp-39-HRP conjugate compete for the anti-gp-39 antibody binding site. Since the number of sites is limited, as more sites are occupied by gp-39 from the sample, fewer sites are left to bind gp-39-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gp-39 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2121310
• NCBI GeneID: 1116
• UniProt Accession #: P36222; P30923; Q8IVA4; Q96HI7; B2R7B0
• Molecular Weight: 42,625 Da
• NCBI Official Full Name: GP-39 cartilage protein
• NCBI Official Synonym Full Names: chitinase 3-like 1 (cartilage glycoprotein-39)
• NCBI Official Symbol: CHI3L1
• NCBI Official Synonym Symbols: GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
• NCBI Protein Information: chitinase-3-like protein 1; 39 kDa synovial protein; cartilage glycoprotein 39
• UniProt Protein Name: Chitinase-3-like protein 1
• UniProt Gene Name: CHI3L1
• UniProt Synonym Gene Names: CGP-39; GP-39; hCGP-39
• UniProt Entry Name: CH3L1_HUMAN

NCBI Description

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. Ref.8

Subunit structure: Monomer.

Subcellular location: Secreted › extracellular space Ref.8.

Tissue specificity: Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. Ref.8

Post-translational modification: Glycosylated. Ref.9

Involvement in disease: Asthma-related traits 7 (ASRT7) [MIM:611960]: Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy and atopic dermatitis.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.

Sequence similarities: Belongs to the glycosyl hydrolase 18 family.

Caution: Although it belongs to the glycosyl hydrolase 18 family, Leu-140 is present instead of the conserved Glu which is an active site residue. Therefore this protein lacks chitinase activity.