GOT1 recombinant protein

Recombinant GOT1 (AST1) protein

Cat. Num. AAA389342

• Gene Names: GOT1; cCAT; GIG18; cAspAT; ASTQTL1
• Synonyms: GOT1; Recombinant GOT1 (AST1) protein; glutamic-oxaloacetic transaminase 1; GOT1 recombinant protein

• Host: E Coli
• Form/Format: Recombinant GOT1/AST1 protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant GOT1 / AST1 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 47.3 kDa Purity: >95%)

Related Product Information for GOT1 recombinant protein

Short Description: Full-length human GOT1/AST1 protein was expressed in E Coli (accession number NP_002070.1) with a C-terminal 6xHis-tag. The molecular weight of the protein is 47.3 kDa.

Background: GOT1 (Glutamic-Oxaloacetic Transaminase 1), also known as AST1, is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic. GOT1 plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. GOT1 catalyzes the reversible conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate in amino acid metabolism and is found in tissues of liver, heart, skeletal muscle, brain, and red blood cells. The enzyme is commonly used as a clinical marker since the serum level of GOT1 indicates the severity of damaged liver and heart tissue. GOT1 expression correlates with tumor growth due to use of the glutamine by various cancer cells. GOT1 is a homodimer where each monomer comprises a small and large domain. Two identical PLP binding sites are located at the dimeric interface between the small and large domains of each monomer. Residue K259 in the catalytic pocket is essential for the formation of the PLP-K259 aldimine and the R387/R293 substrate binding motif.

Product Categories/Family for GOT1 recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4504067
• NCBI GeneID: 2805
• NCBI Accession #: NP_002070.1
• NCBI GenBank Nucleotide #: NM_002079.2
• UniProt Accession #: P17174; Q5VW80; B2R6R7; B7Z7E9
• Molecular Weight: 44,147 Da
• NCBI Official Full Name: aspartate aminotransferase, cytoplasmic
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1, soluble
• NCBI Official Symbol: GOT1
• NCBI Official Synonym Symbols: cCAT; GIG18; cAspAT; ASTQTL1
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic; aspartate aminotransferase 1; aspartate transaminase 1; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; growth-inhibiting protein 18; transaminase
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Protein transport protein
• UniProt Gene Name: GOT1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Uniprot Description

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; Transferase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.3

Chromosomal Location of Human Ortholog: 10q24.1-q25.1

Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol

Molecular Function: cysteine transaminase activity; phosphatidylserine decarboxylase activity; carboxylic acid binding; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; glucose metabolic process; aspartate biosynthetic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; methionine salvage; cellular response to insulin stimulus; sulfur amino acid metabolic process; glycerol biosynthetic process; fatty acid homeostasis; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; polyamine metabolic process

Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1