Rat glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) ELISA Kit | GOT1 elisa kit

Rat Aspartate Aminotransferase, AST ELISA Kit

Cat. Num. AAA704087

• Reactivity: Rat
• Synonyms: glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1); Rat Aspartate Aminotransferase; AST ELISA Kit; Rat Aspartate Aminotransferase (AST) ELISA kit; GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; GOT1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat AST. No significant cross-reactivity or interference between rat AST and analogues was observed.
• Sequence Length: 31

• Samples: Serum, plasma
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12 mIU/ml-200 mIU/ml
• Sensitivity: < 0.78 mIU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GOT1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AST bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126302509
• UniProt Accession #: P12343
• Molecular Weight: 3,427 Da
• NCBI Official Full Name: Aspartate aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Protein transport protein
• UniProt Gene Name: GOT1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_RABIT

Uniprot Description

Function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain

Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.

Cofactor: Pyridoxal phosphate.