Aspartate Aminotransferase Active Protein | GOT1 active protein

Glutamate Oxaloacetate Transmainase

Cat. Num. AAA173082

• Gene Names: GOT1; cCAT; CASPAT
• Purity: Purified
• Synonyms: Aspartate Aminotransferase; Glutamate Oxaloacetate Transmainase; GOT; Aspartate Transaminase; AST; GOT1 active protein

• Host: Porcine heart
• Purity/Purification: Purified
• Form/Format: Powder, lyophilized from sodium phosphate, pH7.0 with pyridoxal-5-phosphate yellow to yellow-green powder
• Sequence Length: 413

• EC Number: 2.6.1.1
• Protein: 0.75 mg protein/mg solid (Coomassie),1.1 mg protein/mg sollid (A₂₈₀)
• Activity: 390 U/mg (Dimension Clinical Chemistry System)
• Unit Definition: One unit will catalyze the transamination of one miromole of L-aspartate to (ALPHA)-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8
• Specific activity: 520 U/mg protein (Coomasie), 350 U/mg protein A (₂₈₀)
• Appearance: Yellow to yellow-green powder
• Contaminants: CPK: <=0.1%; GLDH: <=0.1; GPT/ALT: <= 0.1%; LDH: < 0.1%; MDH: < 0.01%; Protease: < 0.1% (Equivalent to <0.001 O.D /8 min)
• Ammonia: < 0.005 micromole/mg (<0.01%)
• Reconstitution: >= 1 mg/ml in tris buffered saline, 1% BSA, pH 8.0
• Handling: Handle as a potentially hazardous substance.
• Preparation and Storage: Ships on blue ice. Store at -20°C. Aliquot and freeze at -20°C after reconstitution.; ; Storage Note: Protect from moisture.

Related Product Information for GOT1 active protein

MyBioSource produces Porcine Heart Aspartate Transferase (AST), also known as Glutamate Oxaloacetate Transaminase (GOT) for research and manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available. Single batch sizes from 100,000 kU.

There are two SGOT/AST isoenzymes are present in humans, they have high similarity. GOT1, the cytosolic isoenzyme derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver.

Product Categories/Family for GOT1 active protein

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Aspartate Aminotransferase (ast; Got)

NCBI and Uniprot Product Information

• NCBI GI #: 112976
• NCBI GeneID: 396967
• UniProt Accession #: P00503
• NCBI Official Full Name: Aspartate aminotransferase, cytoplasmic
• NCBI Official Symbol: GOT1
• NCBI Official Synonym Symbols: cCAT; CASPAT
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic; transaminase A; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; cysteine aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Aspartate aminotransferase
• UniProt Gene Name: GOT1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_PIG

Uniprot Description

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain ().