Rabbit anti-Human Uracil DNA Glycosylase Polyclonal Antibody | anti-UNG antibody

Uracil DNA Glycosylase, NT (UDG, UNG, DGU, UNG1, UNG15)

Cat. Num. AAA645166

• Gene Names: UNG; ATUNG; UNG; uracil dna glycosylase
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Uracil DNA Glycosylase; Polyclonal Antibody; NT (UDG; UNG; DGU; UNG1; UNG15); Anti -Uracil DNA Glycosylase; anti-UNG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human UNG1. Species sequence homology: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-UNG antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 13aa from human UNG1 at N-terminal (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with UNG antibody at 5ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-UNG antibody

The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG). These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of alternative splicing. UNG is responsible for the removal of uracil from DNA by hydrolysis of the N-glycosidic bond that links the base to the deoxyribose backbone, leaving an abasic site. UNG is a highly conserved enzyme found in many species.

Product Categories/Family for anti-UNG antibody

Antibodies; Abs to DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 15230152
• NCBI GeneID: 821394
• NCBI Accession #: NP_188493.1
• NCBI GenBank Nucleotide #: NM_112749.4
• UniProt Accession #: Q9LIH6
• Molecular Weight: 36,289 Da
• NCBI Official Full Name: uracil dna glycosylase
• NCBI Official Symbol: UNG
• NCBI Official Synonym Symbols: ATUNG; UNG; uracil dna glycosylase
• NCBI Protein Information: uracil dna glycosylase
• UniProt Protein Name: At3g18630
• Protein Family: Probable uracil-DNA glycosylase
• UniProt Gene Name: UNG
• UniProt Entry Name: Q9LIH6_ARATH

NCBI Description

Encodes a uracil-DNA glycosylase (UDG) involved in a base excision DNA repair pathway in mitochondria.

Uniprot Description

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. More active on U:G, U:T and U:C mispairs than on U:A pairs. Highly specific for uracil and no activity with 5-substituted uracil or cytosine derivatives. Required for initiation of base excision repair (BER) of uracil.