Human Golgi-associated PDZ and coiled-coil motif-containing protein ELISA Kit | GOPC elisa kit

Human Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) ELISA Kit

Cat. Num. AAA282916

• Gene Names: GOPC; CAL; FIG; PIST; GOPC1; dJ94G16.2
• Reactivity: Human
• Synonyms: Golgi-associated PDZ and coiled-coil motif-containing protein; Human Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) ELISA Kit; GOPC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GOPC. No significant cross-reactivity or interference between Human GOPC and analogues was observed.
• Sequence Length: 454

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GOPC elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GOPC in samples. An antibody specific for GOPC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGOPC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOPC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOPC bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 62868213
• NCBI GeneID: 57120
• NCBI Accession #: NP_001017408.1
• NCBI GenBank Nucleotide #: NM_001017408.2
• UniProt Accession #: Q9HD26; Q59FS4; Q969U8; A6NM30
• Molecular Weight: 35,130 Da
• NCBI Official Full Name: Golgi-associated PDZ and coiled-coil motif-containing protein isoform b
• NCBI Official Synonym Full Names: golgi associated PDZ and coiled-coil motif containing
• NCBI Official Symbol: GOPC
• NCBI Official Synonym Symbols: CAL; FIG; PIST; GOPC1; dJ94G16.2
• NCBI Protein Information: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Protein Name: Golgi-associated PDZ and coiled-coil motif-containing protein
• Protein Family: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Gene Name: GOPC
• UniProt Synonym Gene Names: CAL; FIG; PIST

NCBI Description

This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: cell junction; cytoplasm; dendrite; Golgi apparatus; Golgi membrane; lysosomal membrane; membrane; plasma membrane; postsynaptic density; postsynaptic membrane; protein complex; trans-Golgi network transport vesicle

Molecular Function: frizzled binding; GTPase regulator activity; ion channel binding; protein binding; protein C-terminus binding; protein homodimerization activity

Biological Process: apical protein localization; cytoplasmic sequestering of CFTR protein; ER to Golgi vesicle-mediated transport; Golgi to plasma membrane transport; negative regulation of anion channel activity; negative regulation of protein localization to cell surface; protein homooligomerization; protein transport; regulation of catalytic activity; spermatid nuclear differentiation

Disease: Spermatogenic Failure 6