GOLPH3 / Golgi Phosphoprotein 3 Recombinant Protein | GOLPH3 recombinant protein

Human GOLPH3 / Golgi Phosphoprotein 3 Recombinant Protein

• Gene Names: GOLPH3; GOPP1; GPP34; MIDAS; Vps74
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: GOLPH3 / Golgi Phosphoprotein 3; Human GOLPH3 / Golgi Phosphoprotein 3 Recombinant Protein; Golgi phosphoprotein 3; Coat protein GPP34; Mitochondrial DNA absence factor; MIDAS; GPP34; GOLPH3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 298

• Applicable Applications for GOLPH3 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Homodimer. Interacts with the coatomer complex. Interacts with MYO18A; the interaction is direct and may link Golgi membranes to the actin cytoskeleton. Interacts with GCNT1; may control its retention in the Golgi. Interacts with VPS35.
• Entry Function: Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 15082415
• NCBI GeneID: 64083
• UniProt Accession #: Q9H4A6
• NCBI Official Full Name: Golgi phosphoprotein 3 (coat-protein)
• NCBI Official Synonym Full Names: golgi phosphoprotein 3
• NCBI Official Symbol: GOLPH3
• NCBI Official Synonym Symbols: GOPP1; GPP34; MIDAS; Vps74
• NCBI Protein Information: Golgi phosphoprotein 3
• UniProt Protein Name: Golgi phosphoprotein 3
• Protein Family: Golgi phosphoprotein
• UniProt Gene Name: GOLPH3
• UniProt Synonym Gene Names: GPP34; MIDAS
• UniProt Entry Name: GOLP3_HUMAN

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

GOLPH3: Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. Belongs to the GOLPH3/VPS74 family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: nucleoplasm; Golgi apparatus; Golgi cisterna; mitochondrion; plasma membrane; mitochondrial intermembrane space; trans-Golgi network; cytosol; endosome

Molecular Function: protein binding; enzyme binding

Biological Process: lamellipodium biogenesis; cell proliferation; cell migration; positive regulation of protein secretion; glycoprotein biosynthetic process; Golgi to plasma membrane protein transport; protein secretion; gene expression; Golgi vesicle budding; leukocyte tethering or rolling; protein retention in Golgi; Golgi organization and biogenesis; positive regulation of TOR signaling pathway