Goat Anti-Mouse IgE Secondary Antibody | IgE secondary antibody
Goat Anti-Mouse IgE-PE
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoblot, Western Blot, Immunoprecipitation
Purity
Affinity chromatography on mouse IgE covalently linked to agarose
Synonyms
Goat Anti-Mouse IgE; Goat Anti-Mouse IgE-PE; IgE secondary antibody
Host
Goat
Isotype
IgG
Specificity
Reacts with the heavy chain of mouse IgE
Purity/Purification
Affinity chromatography on mouse IgE covalently linked to agarose
Concentration
0.5 mg/mL (varies by lot)
Applicable Applications for IgE secondary antibody
ELISA (EIA), FLISA, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoblot (IB), Immunoprecipitation (IP)
Application Notes
Quality tested applications for relevant formats include - ELISA; FLISA; Other referenced applications for relevant formats include -; Flow Cytometry; Immunohistochemistry; Immunoblotting; Immunoprecipitation
Cross Adsorption
Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA; may react with immunoglobulins from other species
Buffer Formulation
Supplied in PBS/NaN3 and a stabilizing agent
Isotype Control
Goat IgG-PE
Preparation and Storage
Store at 2-8 degree C
References
1. Costa-Pinto FA, Basso AS, Britto LR, Malucelli BE, Russo M. Avoidance behavior and neural correlates of allergen exposure in a murine model of asthma. Brain Behav Immun. 2005;19:52-60. (ELISA)
2. Andrade MC, Menezes JS, Cassali GD, Martins-Filho OA, Cara DC, Faria AM. Alcohol-induced gastritis prevents oral tolerance induction in mice. Clin Exp Immunol. 2006;146:312-22. (ELISA)
3. Prescott VE, Forbes E, Foster PS, Matthaei K, Hogan SP. Mechanistic analysis of experimental food allergen-induced cutaneous reactions. J Leukoc Biol. 2006;80:258-66. (ELISA)
4. Arnaboldi PM, Roth-Walter F, Mayer L. Suppression of Th1 and Th17, but not Th2, responses in a CD8+ T cell mediated model of oral tolerance. Mucosal Immunol. 2009;2:427-38. (ELISA)
5. Paveglio S, Puddington L, Rafti E, Matson AP. FcRn-mediated intestinal absorption of IgG anti-IgE/IgE immune complexes in mice. Clin Exp Immunol. 2012;42:1791-800. (ELISA)
6. Kawabe Y, Hayashida Y, Numata K, Harada S, Hayashida Y, Ito A, et al. Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens. PLoS One. 2012;7(10):e48512. (ELISA)
7. Kin NW, Stefanov EK, Dizon BL, Kearney JF. Antibodies generated against conserved antigens expressed by bacteria and allergen-bearing fungi suppress airway disease. J Immunol. 2012;189:2246-56. (ELISA)
8. Lochmatter C, Schneider CL, Ingram K, Keiser J, Schifferli JA. Schistosoma mansoni tetraspanning orphan receptor (SmTOR): a new vaccine candidate against schistosomiasis. Clin Exp Immunol. 2012;170:342-57. (ELISA)
9. Durham CG, Schwiebert LM, Lorenz RG. Use of the cockroach antigen model of acute asthma to determine the immunomodulatory role of early exposure to gastrointestinal infection. Methods Mol Biol. 2013;1032:271-86. (ELISA)
10. Vukcevic M, Zorzato F, Keck S, Tsakiris DA, Keiser J, Maizels RM, et al. Gain of function in the immune system caused by a ryanodine receptor 1 mutation. J Cell Sci. 2013;126:3485-92. (ELISA)
11. Brachs S, Turqueti-Neves A, Stein M, Reimer D, Brachvogel B, Bösl M, et al. Swiprosin-1/EFhd2 limits germinal center responses and humoral type 2 immunity. Eur J Immunol. 2014;44:3206-19. (ELISA)
12. Zhou W, Goleniewska K, Zhang J, Dulek DE, Toki S, Lotz MT, et al. Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling. J Allergy Clin Immunol. 2014;134:698-705. (ELISA)
13. Joshi VB, Adamcakova-Dodd A, Jing X, Wongrakpanich A, Gibson-Corley KN, Thorne PS, et al. Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy. AAPS J. 2014;16:975-85. (ELISA)
14. Sun R, Tang X, Yao H, Hong S, Yang Y, Kou W, et al. Establishment of a new animal model of allergic rhinitis with biphasic sneezing by intranasal sensitization with Staphylococcal enterotoxin B. Exp Ther Med. 2015;10:407-12. (ELISA)
15. Bracken SJ, Adami AJ, Szczepanek SM, Ehsan M, Natarajan P, Guernsey LA, et al. Long-term exposure to house dust mite leads to the suppression of allergic airway disease despite persistent lung inflammation. Int Arch Allergy Immunol. 2015;166:243-58. (ELISA)
16. Patel PS, Kearney JF. Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life. J Immunol. 2015;194:5838-50. (ELISA)
17. Chen X, Lin J, Pan T, Cao M, Shi C, Cai Q, et al. Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay. Fish Sci. 2015;81:551-8. (ELISA)
18. Vogel L, Lüttkopf D, Hatahet L, Haustein D, Vieths S. Development of a functional in vitro assay as a novel tool for the standardization of allergen extracts in the human system. Allergy. 2005;60:1021-8. (FC)
19. Samarasinghe AE, Hoselton SA, Schuh JM. The absence of VPAC2 leads to aberrant antibody production in Aspergillus fumigatus sensitized and challenged mice. Peptides. 2011;32:131-7. (IHC)
20. Burns TA, Westerman T, Nuovo GJ, Watts MR, Pettigrew A, Yin C, et al. Role of oxidative tissue injury in the pathophysiology of experimentally induced equine laminitis: a comparison of 2 models. J Vet Intern Med. 2011;25:540-8. (WB)
21. Nieuwenhuizen NE, Meter JM, Horsnell WG, Hoving JC, Fick L, Sharp MF, et al. A cross-reactive monoclonal antibody to nematode haemoglobin enhances protective immune responses to Nippostrongylus brasiliensis. PLoS Negl Trop Dis. 2013;7(8):e2395. (WB)
22. Hazenbos WL, Wu P, Eastham-Anderson J, Kinoshita T, Brown EJ. Impaired FcepsilonRI stability, signaling, and effector functions in murine mast cells lacking glycosylphosphatidylinositol-anchored proteins. Blood. 2011;118:4377-83. (IP)
2. Andrade MC, Menezes JS, Cassali GD, Martins-Filho OA, Cara DC, Faria AM. Alcohol-induced gastritis prevents oral tolerance induction in mice. Clin Exp Immunol. 2006;146:312-22. (ELISA)
3. Prescott VE, Forbes E, Foster PS, Matthaei K, Hogan SP. Mechanistic analysis of experimental food allergen-induced cutaneous reactions. J Leukoc Biol. 2006;80:258-66. (ELISA)
4. Arnaboldi PM, Roth-Walter F, Mayer L. Suppression of Th1 and Th17, but not Th2, responses in a CD8+ T cell mediated model of oral tolerance. Mucosal Immunol. 2009;2:427-38. (ELISA)
5. Paveglio S, Puddington L, Rafti E, Matson AP. FcRn-mediated intestinal absorption of IgG anti-IgE/IgE immune complexes in mice. Clin Exp Immunol. 2012;42:1791-800. (ELISA)
6. Kawabe Y, Hayashida Y, Numata K, Harada S, Hayashida Y, Ito A, et al. Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens. PLoS One. 2012;7(10):e48512. (ELISA)
7. Kin NW, Stefanov EK, Dizon BL, Kearney JF. Antibodies generated against conserved antigens expressed by bacteria and allergen-bearing fungi suppress airway disease. J Immunol. 2012;189:2246-56. (ELISA)
8. Lochmatter C, Schneider CL, Ingram K, Keiser J, Schifferli JA. Schistosoma mansoni tetraspanning orphan receptor (SmTOR): a new vaccine candidate against schistosomiasis. Clin Exp Immunol. 2012;170:342-57. (ELISA)
9. Durham CG, Schwiebert LM, Lorenz RG. Use of the cockroach antigen model of acute asthma to determine the immunomodulatory role of early exposure to gastrointestinal infection. Methods Mol Biol. 2013;1032:271-86. (ELISA)
10. Vukcevic M, Zorzato F, Keck S, Tsakiris DA, Keiser J, Maizels RM, et al. Gain of function in the immune system caused by a ryanodine receptor 1 mutation. J Cell Sci. 2013;126:3485-92. (ELISA)
11. Brachs S, Turqueti-Neves A, Stein M, Reimer D, Brachvogel B, Bösl M, et al. Swiprosin-1/EFhd2 limits germinal center responses and humoral type 2 immunity. Eur J Immunol. 2014;44:3206-19. (ELISA)
12. Zhou W, Goleniewska K, Zhang J, Dulek DE, Toki S, Lotz MT, et al. Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling. J Allergy Clin Immunol. 2014;134:698-705. (ELISA)
13. Joshi VB, Adamcakova-Dodd A, Jing X, Wongrakpanich A, Gibson-Corley KN, Thorne PS, et al. Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy. AAPS J. 2014;16:975-85. (ELISA)
14. Sun R, Tang X, Yao H, Hong S, Yang Y, Kou W, et al. Establishment of a new animal model of allergic rhinitis with biphasic sneezing by intranasal sensitization with Staphylococcal enterotoxin B. Exp Ther Med. 2015;10:407-12. (ELISA)
15. Bracken SJ, Adami AJ, Szczepanek SM, Ehsan M, Natarajan P, Guernsey LA, et al. Long-term exposure to house dust mite leads to the suppression of allergic airway disease despite persistent lung inflammation. Int Arch Allergy Immunol. 2015;166:243-58. (ELISA)
16. Patel PS, Kearney JF. Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life. J Immunol. 2015;194:5838-50. (ELISA)
17. Chen X, Lin J, Pan T, Cao M, Shi C, Cai Q, et al. Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay. Fish Sci. 2015;81:551-8. (ELISA)
18. Vogel L, Lüttkopf D, Hatahet L, Haustein D, Vieths S. Development of a functional in vitro assay as a novel tool for the standardization of allergen extracts in the human system. Allergy. 2005;60:1021-8. (FC)
19. Samarasinghe AE, Hoselton SA, Schuh JM. The absence of VPAC2 leads to aberrant antibody production in Aspergillus fumigatus sensitized and challenged mice. Peptides. 2011;32:131-7. (IHC)
20. Burns TA, Westerman T, Nuovo GJ, Watts MR, Pettigrew A, Yin C, et al. Role of oxidative tissue injury in the pathophysiology of experimentally induced equine laminitis: a comparison of 2 models. J Vet Intern Med. 2011;25:540-8. (WB)
21. Nieuwenhuizen NE, Meter JM, Horsnell WG, Hoving JC, Fick L, Sharp MF, et al. A cross-reactive monoclonal antibody to nematode haemoglobin enhances protective immune responses to Nippostrongylus brasiliensis. PLoS Negl Trop Dis. 2013;7(8):e2395. (WB)
22. Hazenbos WL, Wu P, Eastham-Anderson J, Kinoshita T, Brown EJ. Impaired FcepsilonRI stability, signaling, and effector functions in murine mast cells lacking glycosylphosphatidylinositol-anchored proteins. Blood. 2011;118:4377-83. (IP)
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Product Notes
The IgE (Catalog #AAA670682) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Goat Anti-Mouse IgE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), FLISA, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoblot (IB), Immunoprecipitation (IP). Quality tested applications for relevant formats include - ELISA; FLISA; Other referenced applications for relevant formats include -; Flow Cytometry; Immunohistochemistry; Immunoblotting; Immunoprecipitation. Researchers should empirically determine the suitability of the IgE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Mouse IgE, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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