Dot Blot
(Shown here is a serial 1:1 dilution of Rabbit IgG protein (250ng starting total load) co-incubated with Goat anti Rabbit IgG (HRP) and Goat anti Rabbit (Dylight 649) 1:20,000. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.)
Goat anti Rabbit IgG (H + L) (HRP) Secondary Antibody | IgG secondary antibody
Goat anti Rabbit IgG (H + L) (HRP)
Reactivity
Rabbit IgG (H + L)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Goat anti Rabbit IgG (H + L) (HRP) was purified by affinity chromatography.
Synonyms
Goat anti Rabbit IgG (H + L) (HRP); Goat anti Rabbit Immunoglobulin G (H + L) secondary (HRP); IgG secondary antibody
Host
Goat
Reactivity
Rabbit IgG (H + L)
Specificity
Rabbit IgG (H + L)
Purity/Purification
Goat anti Rabbit IgG (H + L) (HRP) was purified by affinity chromatography.
Form/Format
Lyophilized from 0.02M K3PO4, pH 7.2 with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamicin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.
Applicable Applications for IgG secondary antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:90,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000
Tag/Conjugate
HRP
Immunogen
Goat anti-rabbit IgG (H+L) (HRP) was raised in goat using rabbit IgG, whole molecule as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Dot Blot
(Shown here is a serial 1:1 dilution of Rabbit IgG protein (250ng starting total load) co-incubated with Goat anti Rabbit IgG (HRP) and Goat anti Rabbit (Dylight 649) 1:20,000. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.)
Dot Blot
(Shown here is a serial 1:1 dilution of Rabbit IgG protein (250ng starting total load) co-incubated with Goat anti Rabbit IgG (HRP) and Goat anti Rabbit (Dylight 649) 1:20,000. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.)
Western Blot (WB)
(Goat Anti Rabbit IgG (HRP)secondary antibody was used at 1:40,000 to detect a rabbit primary antibody by Western Blot. p27 antibody (1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone.)
Western Blot (WB)
(Goat Anti Rabbit IgG (HRP)secondary antibody was used at 1:40,000 to detect a rabbit primary antibody by Western Blot. p27 antibody (1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone.)
Related Product Information for IgG secondary antibody
Goat anti Rabbit IgG (H + L) secondary antibody (HRP)
Product Categories/Family for IgG secondary antibody
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Product Notes
The IgG (Catalog #AAA538597) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Rabbit IgG (H + L) (HRP) reacts with Rabbit IgG (H + L) and may cross-react with other species as described in the data sheet. AAA Biotech's Goat anti Rabbit IgG (H + L) (HRP) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:90,000 IHC: 1:1,000-1:5,000 WB: 1:2,000-1:10,000. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat anti Rabbit IgG (H + L) (HRP), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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