Western Blot (WB)
(Western Blot analysis of cell extracts from untransfected NIH/3T3 cells (lane 1) or NIH/3T3 cells transfected with a plasmid encoding mMCP-8-DYKDDDDK tagged protein (lane 2), using MBS6010758.)
Rat anti-Mouse MCP-8 Monoclonal Antibody | anti-Cd46 antibody
MCP-8 (Mast Cell Protease 8, Mcpt8)
Gene Names
Cd46; Mcp
Reactivity
Mouse
Applications
Western Blot
Purity
Purified by affinity chromatography.
Synonyms
MCP-8; Monoclonal Antibody; MCP-8 (Mast Cell Protease 8; Mcpt8); Anti -MCP-8 (Mast Cell Protease 8; anti-Cd46 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Specificity
Recognizes mouse Mast Cell Protease 8.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-Cd46 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1-2ug/ml
Dilution: Western Blot: 1-2ug/ml
Immunogen
Recombinant GST-mouse mMCP-8 fusion protein expressed in E.coli.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of cell extracts from untransfected NIH/3T3 cells (lane 1) or NIH/3T3 cells transfected with a plasmid encoding mMCP-8-DYKDDDDK tagged protein (lane 2), using MBS6010758.)
Western Blot (WB)
(Western Blot analysis of cell extracts from untransfected NIH/3T3 cells (lane 1) or NIH/3T3 cells transfected with a plasmid encoding mMCP-8-DYKDDDDK tagged protein (lane 2), using MBS6010758.)
Related Product Information for anti-Cd46 antibody
mMCP-8 and its rat homologues, rMCP-8, -9, and -10, form a new group of mast cell/basophil proteases, which are more closely related to the T-cell granzymes and neutrophil cathepsin G than to the mast cell tryptases and chymases. mMCP-8 showed a high degree of homology with mouse granzyme B in the critical regions for determining substrate cleavage specificity. It preferentially cleaves after Asp residues. mMCP-8 mRNA is highly expressed in a mouse connective tissue MC-like tumor line. However, it could not be detected in mouse liver, intestine, lung or ears, indicating very low expression in normal tissues. A strong increase in mMCP-8 levels in the lungs can be detected in infected animals.
Product Categories/Family for anti-Cd46 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
membrane cofactor protein
NCBI Official Synonym Full Names
CD46 molecule, complement regulatory protein
NCBI Official Symbol
Cd46
NCBI Official Synonym Symbols
Mcp
NCBI Protein Information
membrane cofactor protein; CD46 antigen, complement regulatory protein
UniProt Protein Name
Membrane cofactor protein
Protein Family
UniProt Gene Name
Cd46
UniProt Synonym Gene Names
Mcp
UniProt Entry Name
MCP_RAT
NCBI Description
may be involved in fertilization or reproduction; human homolog acts as an inhibitor of plasma membrane complement [RGD, Feb 2006]
Uniprot Description
Function: May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Ref.2 Ref.3
Subcellular location: Cytoplasmic vesicle › secretory vesicle › acrosome inner membrane; Single-pass membrane protein. Note: Inner acrosomal membrane of spermatozoa. Ref.3
Tissue specificity: Specifically expressed in testis. Within testis, present only in elongated spermatids and spermatozoa (at protein level). Ref.1 Ref.2 Ref.3
Developmental stage: Not expressed in embryonic and immature rats. Expressed in parallel with synthesis of spermatids. Ref.3
Post-translational modification: O-glycosylated. Ref.3N-glycosylated. Ref.3
Sequence similarities: Contains 4 Sushi (CCP/SCR) domains.
Similar Products
Product Notes
The Cd46 cd46 (Catalog #AAA6010758) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The MCP-8 (Mast Cell Protease 8, Mcpt8) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MCP-8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1-2ug/ml. Researchers should empirically determine the suitability of the Cd46 cd46 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCP-8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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