Western Blot (WB)
(MBS423108 (1ug/ml) staining of U937 (A) and (0.3ug/ml) A431 (B) and HepG2 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Goat OSMR Antibody | anti-OSMR antibody
Goat anti-OSMR Antibody
Western blot: Approx 125kDa band observed in lysates of cell line U937 and approx 70+125kDa in lysates of cell line A431 and HepG2 (calculated MW of 111kDa according to NP_003990.1 (isoform 1) and NP_001310435.1 (isoform 3), and 66.0kDa according to NP_001310436.1 (isoform 4)). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Western Blot (WB)
(MBS423108 (1ug/ml) staining of U937 (A) and (0.3ug/ml) A431 (B) and HepG2 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Western Blot (WB)
(MBS423108 (1ug/ml) staining of U937 (A) and (0.3ug/ml) A431 (B) and HepG2 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Flow Cytometry (FC/FACS)
(MBS423108 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(MBS423108 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the type I cytokine receptor family. The encoded protein heterodimerizes with interleukin 6 signal transducer to form the type II oncostatin M receptor and with interleukin 31 receptor A to form the interleukin 31 receptor, and thus transduces oncostatin M and interleukin 31 induced signaling events. Mutations in this gene have been associated with familial primary localized cutaneous amyloidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]
Uniprot Description
OSMR: Associates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events. Defects in OSMR are the cause of amyloidosis primary localized cutaneous type 1 (PLCA1); also known as familial lichen amyloidosis or familial cutaneous lichen amyloidosis. PLCA1 is a hereditary primary amyloidosis characterized by localized cutaneous amyloid deposition. This condition usually presents with itching (especially on the lower legs) and visible changes of skin hyperpigmentation and thickening (lichenification) that may be exacerbated by chronic scratching and rubbing. The amyloid deposits probably reflect a combination of degenerate keratin filaments, serum amyloid P component, and deposition of immunoglobulins. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 5p13.1
Cellular Component: apical plasma membrane; oncostatin-M receptor complex; plasma membrane
Molecular Function: growth factor binding; oncostatin-M receptor activity
Biological Process: positive regulation of acute inflammatory response; positive regulation of cell proliferation; response to cytokine stimulus
Disease: Amyloidosis, Primary Localized Cutaneous, 1
Research Articles on OSMR
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Product Notes
The OSMR osmr (Catalog #AAA423108) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-OSMR Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's OSMR can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA, Western Blot (WB), Flow Cytometry (FC/FACS). Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 125kDa band observed in lysates of cell line U937 and approx 70+125kDa in lysates of cell line A431 and HepG2 (calculated MW of 111kDa according to NP_003990.1 (isoform 1) and NP_001310435.1 (isoform 3), and 66.0kDa according to NP_001310436.1 (isoform 4)). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the OSMR osmr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OSMR, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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