Mouse Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) ELISA Kit | DSTYK elisa kit

Mouse Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) ELISA Kit

Cat. Num. AAA457022

• Gene Names: Dstyk; Ripk5; C820013G01; A930019K20Rik; C430014H23Rik
• Reactivity: Mouse
• Synonyms: Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK); Mouse Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) ELISA Kit; RIPK5; Dusty PK; RIP5; SgK496; Receptor Interacting Protein Kinase 5; RIP-homologous kinase; Sugen kinase 496; Receptor-interacting serine/threonine-protein kinase 5; DSTYK elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DSTYK. No significant cross-reactivity or interference between DSTYK and analogues was observed.
• Sequence Length: 927

• Samples: Tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.063ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DSTYK were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DSTYK were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DSTYK elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of DSTYK in mouse tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DSTYK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DSTYK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DSTYK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DSTYK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 40556610
• NCBI GeneID: 213452
• NCBI Accession #: NP_766104.2
• NCBI GenBank Nucleotide #: NM_172516.4
• UniProt Accession #: Q6XUX1; Q3ULK4; Q5EBN5; Q8C7D4; Q8C923; Q9CTP7
• Molecular Weight: 18,814 Da
• NCBI Official Full Name: dual serine/threonine and tyrosine protein kinase
• NCBI Official Synonym Full Names: dual serine/threonine and tyrosine protein kinase
• NCBI Official Symbol: Dstyk
• NCBI Official Synonym Symbols: Ripk5; C820013G01; A930019K20Rik; C430014H23Rik
• NCBI Protein Information: dual serine/threonine and tyrosine protein kinase
• UniProt Protein Name: Dual serine/threonine and tyrosine protein kinase
• Protein Family: Dual serine/threonine and tyrosine protein kinase
• UniProt Gene Name: Dstyk
• UniProt Synonym Gene Names: Ripk5; Dusty PK

Uniprot Description

Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation. Involved in the regulation of both caspase-dependent apoptosis and caspase-independent cell death. In the skin, it plays a predominant role in suppressing caspase-dependent apoptosis in response to UV stress in a range of dermal cell types.