Goat OCIL Antibody | anti-OCIL antibody

Goat Anti-OCIL Antibody

Cat. Num. AAA423846

• Gene Names: CLEC2D; CLAX; LLT1; OCIL
• Reactivity: Human; Expected from sequence similarity: Human, Dog, Cow
• Applications: ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: OCIL; Goat Anti-OCIL Antibody; CLEC2D; C-type lectin domain family 2; member D; CLAX; LLT1; C-type lectin related f; C-type lectin superfamily 2; lectin-like NK cell receptor; lectin-like transcript 1; osteoclast inhibitory lectin; anti-OCIL antibody

• Host: Goat
• Reactivity: Human; Expected from sequence similarity: Human, Dog, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 191

• Applicable Applications for anti-OCIL antibody: Peptide ELISA (EIA), WB, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000; WB: Approx 30-35kDa band observed in lysates of histiocytic lymphoma cell line U937 (calculated MW of 22.3kDa according to NP_001004419.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.; IHC: Paraffin embedded Human Tonsil and Placenta. Recommended concentration: 3.75ug/ml.; IF: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10ug/ml.; FC/FACS: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Immunogen: Peptide with sequence C-LLRYKGPSDH, from the Internal region of the protein sequence according to NP_037401.1; NP_001004419.1; NP_001184246.1.
• Peptide Sequence: LLRYKGPSDH
• Epitope: Internal region
• Important comments: This antibody is expected to recognize all isoforms (NP_037401.1; NP_001004419.1; NP_001184246.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain i)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f)

Immunohistochemistry (IHC)

((3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Immunohistochemistry (IHC)

((3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

References

Skinningsrud B, Husebye ES, Pearce SH, McDonald DO, Brandal K, Wolff AB, Løvås K, Egeland T, Undlien DE.: Polymorphisms in CLEC16A and CIITA at 16p13 are associated with primary adrenal insufficiency. J Clin Endocrinol Metab. 2008 Sep;93(9):3310-7. Epub 2008 Jul 1. PMID: 18593762

NCBI and Uniprot Product Information

• NCBI GI #: 7019447
• NCBI GeneID: 29121
• NCBI Accession #: NP_037401.1; NP_001004419.1; NP_001184246.1
• NCBI GenBank Nucleotide #: NP_037401.1
• UniProt Accession #: Q9UHP7
• NCBI Official Full Name: C-type lectin domain family 2 member D isoform 1
• NCBI Official Synonym Full Names: C-type lectin domain family 2 member D
• NCBI Official Symbol: CLEC2D
• NCBI Official Synonym Symbols: CLAX; LLT1; OCIL
• NCBI Protein Information: C-type lectin domain family 2 member D
• UniProt Protein Name: C-type lectin domain family 2 member D
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC2D
• UniProt Synonym Gene Names: CLAX; LLT1; OCIL; LLT-1

NCBI Description

This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis (PubMed:20843815, PubMed:16339513). Inhibits osteoclast formation (PubMed:14753741, PubMed:15123656). Inhibits bone resorption (PubMed:14753741). Modulates the release of interferon-gamma (PubMed:15104121). Binds high molecular weight sulfated glycosaminoglycans (PubMed:15123656).