Goat VE-Cadherin Antibody | anti-CDH5 antibody

Goat Anti-VE-Cadherin (aa537-549) Antibody

Cat. Num. AAA423873

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Human; Expected from sequence similarity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: VE-Cadherin; Goat Anti-VE-Cadherin (aa537-549) Antibody; CDH5; cadherin 5; type 2 (vascular endothelium); 7B4; CD144; 7B4 antigen; VE-cadherin; type 2; VE-cadherin (vascular epithelium); cadherin-5; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; anti-CDH5 antibody

• Host: Goat
• Reactivity: Human; Expected from sequence similarity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 784

• Applicable Applications for anti-CDH5 antibody: Peptide ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000; WB: Preliminary experiments gave an approx 100kDa band in lysates of cell line HeLa and approx. 110kDa in A549 at 1-2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observed, given the calculated size of 87.5kDa according to NP_001786.2.; FC/FACS: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Immunogen: Peptide with sequence C-TVKYGQFDREHTK, from the internal region of the protein sequence according to NP_001786.2.
• Peptide Sequence: TVKYGQFDREHTK
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol)

References

Adam AP, Sharenko AL, Pumiglia K, Vincent PA.: Src-induced tyrosine phosphorylation of VE-cadherin is not sufficient to decrease barrier function of endothelial monolayers. The Journal of biological chemistry 2010 Mar 285 (10): 7045-55. PMID: 20048167

NCBI and Uniprot Product Information

• NCBI GI #: 166362713
• NCBI GeneID: 1003
• NCBI Accession #: NP_001786.2
• NCBI GenBank Nucleotide #: NP_001786.2
• UniProt Accession #: P33151
• NCBI Official Full Name: cadherin-5 preproprotein
• NCBI Official Synonym Full Names: cadherin 5
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5
• UniProt Protein Name: Cadherin-5
• Protein Family: Cadherin
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_HUMAN

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: adherens junction; tight junction; membrane; plasma membrane; integral to membrane; intercellular junction; cell junction

Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation