Peroxiredoxin-SO3 Monoclonal Antibody | anti-SO3 antibody

Mouse Anti-Peroxiredoxin-SO3

Cat. Num. AAA6506768

• Applications: Western Blot
• Purity: Affinity Purified. Purified by Protein G affinity chromatography.
• Synonyms: Peroxiredoxin-SO3; Monoclonal Antibody; Mouse Anti-Peroxiredoxin-SO3; anti-SO3 antibody

• Host: Host: Mouse; Source: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 10A1
• Specificity: Recognizes human Peroxiredoxin-SO3.
• Purity/Purification: Affinity Purified. Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.

• Applicable Applications for anti-SO3 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1.0ug/ml
• Crossreactivity: Human
• Immunogen: Sulfonylated peptide (KLH coupled) corresponding to the active site sequence common to mammalian Prx I to IV
• Positive Control: HeLa cell treated with H2O2
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

Related Product Information for anti-SO3 antibody

Peroxiredoxin(Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at their active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Prx I to IV have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Prx I. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress condition, the sulfenic intermediate(Cys51-SOH) can be easily overoxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that overoxidized Prx can be reduced back to the active form during recovery after oxidative stress. Anti-Prx-SO3 antibody recognizes both sulfinic and sulfonic forms of Prx and detects overoxidized Prx enzymes in H2O2-treated cells with high sensitivity and specificity.