Goat Apolipoprotein B-100 Antibody | anti-APOB antibody

Goat Anti-Human Apolipoprotein B-100 Affinity Pure

Cat. Num. AAA390081

• Gene Names: APOB; FLDB; LDLCQ4
• Applications: ELISA, Immunoblot, Western Blot
• Synonyms: Apolipoprotein B-100; Goat Anti-Human Apolipoprotein B-100 Affinity Pure; Goat Anti-human Apolipoprotein B-100/48 (Apo B-100/48) Polyclonal Antibody; Affinity Purified; anti-APOB antibody

• Host: Goat
• Specificity: Specifically binds to human apo B-100. Dilution for immunoblot and ELISA range: 200 to 80,000.
• Concentration: 1 mg / ml (OD 1.35 / 280 nm). (varies by lot)
• Sequence Length: 4563

• Applicable Applications for anti-APOB antibody: ELISA (EIA), Immunoblot (IB)
• Application Notes: Dilution for Immunoblot & ELISA range: 1,000 to 80,000.; Use: The antibody can be used for detection of apo B-100 in plasma and lipoproteins, immunoassays, immunoblots, enzyme conjugation, or biotinylation.
• Source Note: Goat anti human apo B-100 polyclonal antibody purified by human LDL-Sepharose affinity column.*
• Buffer: 75 mM PBS, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.4.
• Preparation and Storage: -20 degree C for long-term storage, 2-8 degree C for short-term storage. Aliquot to avoid repeated thaw and freeze.

Product Categories/Family for anti-APOB antibody

Aff. Pure AB

NCBI and Uniprot Product Information

• NCBI GI #: 105990532
• NCBI GeneID: 338
• NCBI Accession #: NP_000375.2
• NCBI GenBank Nucleotide #: NM_000384.2
• UniProt Accession #: P04114; O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4
• NCBI Protein Information: apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; apolipoprotein B48; apolipoprotein B (including Ag(x) antigen)
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48
• UniProt Entry Name: APOB_HUMAN

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Subunit structure: Interacts with PCSK9. Ref.38

Subcellular location: Cytoplasm. Secreted Ref.38.

Induction: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level). Ref.34

Post-translational modification: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. Ref.31

Involvement in disease: Familial hypobetalipoproteinemia 1 (FHBL1) [MIM:107730]: A disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.46Familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]: Dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.40 Ref.42 Ref.44 Ref.49Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Sequence similarities: Contains 1 vitellogenin domain.

RNA editing: Edited at position 2180.The stop codon (UAA) at position 2180 is created by RNA editing. Apo B-48, derived from the fully edited RNA, is produced only in the intestine and is found in chylomicrons. Apo B-48 is a shortened form of apo B-100 which lacks the LDL-receptor region. The unedited version (apo B-100) is produced by the liver and is found in the VLDL and LDL.

Sequence caution: The sequence AAA51752.1 differs from that shown. Reason: Frameshift at positions 942, 951, 1139, 1165, 1164, 1371 and 1385.