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Human Gonadotropin-releasing hormone ELISA Kit | GnRH elisa kit

Human Gonadotropin-releasing hormone (GnRH) ELISA Kit

Gene Names
GNRH1; GRH; GNRH; LHRH; LNRH
Reactivity
Human
Synonyms
Gonadotropin-releasing hormone; Human Gonadotropin-releasing hormone (GnRH) ELISA Kit; GnRH elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GNRH1. No significant cross-reactivity or interference between Human GNRH1 and analogues was observed.
Sequence Length
99
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GnRH elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GNRH1 in samples. An antibody specific for GNRH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGNRH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GNRH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GNRH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,380 Da
NCBI Official Full Name
progonadoliberin-1 isoform 2 preproprotein
NCBI Official Synonym Full Names
gonadotropin releasing hormone 1
NCBI Official Symbol
GNRH1
NCBI Official Synonym Symbols
GRH; GNRH; LHRH; LNRH
NCBI Protein Information
progonadoliberin-1
UniProt Protein Name
Progonadoliberin-1
UniProt Gene Name
GNRH1
UniProt Synonym Gene Names
GNRH; GRH; LHRH; GnRH-I; LH-RH I

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism. [provided by RefSeq, Nov 2015]

Uniprot Description

GNRH1: Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. Belongs to the GnRH family.

Protein type: Cell cycle regulation; Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: axon terminus; dendrite; extracellular region; extracellular space; Golgi-associated vesicle; mitochondrion; perikaryon

Molecular Function: gonadotropin hormone-releasing hormone activity; hormone activity

Biological Process: aging; cell-cell signaling; female pregnancy; male sex determination; multicellular organism development; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of immature T cell proliferation; regulation of gene expression; response to corticosteroid stimulus; response to ethanol; response to lipopolysaccharide; response to testosterone stimulus; signal transduction

Disease: Hypogonadotropic Hypogonadism 7 With Or Without Anosmia

Research Articles on GnRH

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Product Notes

The Human GnRH gnrh1 (Catalog #AAA280854) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280854 ELISA Kit recognizes Human GnRH. It is sometimes possible for the material contained within the vial of "Gonadotropin-releasing hormone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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