Guanine nucleotide-binding protein G (t) subunit alpha-3 (GNAT3) Recombinant Protein | GNAT3 recombinant protein

Recombinant Human Guanine nucleotide-binding protein G (t) subunit alpha-3 (GNAT3)

Cat. Num. AAA1006079

• Gene Names: GNAT3; GDCA
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Guanine nucleotide-binding protein G (t) subunit alpha-3 (GNAT3); Recombinant Human Guanine nucleotide-binding protein G (t) subunit alpha-3 (GNAT3); GNAT3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-354, Full length protein
• Sequence: GSGISSESKESAKRSKELEKKLQEDAERDARTVKLLLLGAGESGKSTIVKQMKIIHKNGYSEQECMEFKAVIYSNTLQSILAIVKAMTTLGIDYVNPRSAEDQRQLYAMANTLEDGGMTPQLAEVIKRLWRDPGIQACFERASEYQLNDSAAYYLNDLDRITASGYVPNEQDVLHSRVKTTGIIETQFSFKDLHFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDEEVNRMHESLHLFNSICNHKYFSTTSIVLFLNKKDIFQEKVTKVHLSICFPEYTGPNTFEDAGNYIKNQFLDLNLKKEDKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF
• Sequence Length: 353

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 156139155
• NCBI GeneID: 346562
• NCBI Accession #: NP_001095856.1
• NCBI GenBank Nucleotide #: NM_001102386.2
• UniProt Accession #: A8MTJ3; A4D1B2; A4D1B3; B9EJG5
• Molecular Weight: 40,357 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(t) subunit alpha-3
• NCBI Official Synonym Full Names: G protein subunit alpha transducin 3
• NCBI Official Symbol: GNAT3
• NCBI Official Synonym Symbols: GDCA
• NCBI Protein Information: guanine nucleotide-binding protein G(t) subunit alpha-3
• UniProt Protein Name: Guanine nucleotide-binding protein G(t) subunit alpha-3
• UniProt Gene Name: GNAT3

NCBI Description

Sweet, bitter, and umami tastes are transmitted from taste receptors by a specific guanine nucleotide binding protein. The protein encoded by this gene is the alpha subunit of this heterotrimeric G protein, which is found not only in the oral epithelium but also in gut tissues. Variations in this gene have been linked to metabolic syndrome. [provided by RefSeq, Dec 2015]

Uniprot Description

Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.