Rabbit anti-Human MBD2 Polyclonal Antibody | anti-MBD2 antibody

Anti-MBD2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103274

• Gene Names: MBD2; DMTase; NY-CO-41
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MBD2; Polyclonal Antibody; Anti-MBD2 Antibody; Rabbit Polyclonal; MBD2 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-MBD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MBD2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MBD2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human MBD2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human MBD2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MBD2 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa)

Related Product Information for anti-MBD2 antibody

MBD (methyl-CpG-binding domain) proteins selectively bind to methylated DNA and recruit chromatin remodelling and transcriptional repressor complexes, thereby establishing a repressive chromatin state. MBD2, a member of the MBD protein family, binds to methylated promoter CpG islands (clusters of high-density CpG dinucleotides) and acts as a methylation-dependent transcriptional repressor.MBD2, a methyl-CpG-binding protein, is a component of the MeCP1 histone deacetylase (HDAC) complex and plays a critical role in DNA methylation-mediated transcriptional repression. Methyl-CpG-binding domain protein 2 (MBD2) plays an important role in diverse immunological disorders by regulating immune cell functions, such as differentiation and mediator secretion. Methyl-CpG binding domain 2 (MBD2) leads to the silencing of methylated genes in cancer cells and was implicated in the activation of prometastatic genes in hepatocellular carcinoma (HCC).

NCBI and Uniprot Product Information

• NCBI GI #: 50401198
• NCBI GeneID: 8932
• UniProt Accession #: Q9UBB5; O95242; Q9UIS8
• Molecular Weight: 411
• NCBI Official Full Name: Methyl-CpG-binding domain protein 2
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 2
• NCBI Official Symbol: MBD2
• NCBI Official Synonym Symbols: DMTase; NY-CO-41
• NCBI Protein Information: methyl-CpG-binding domain protein 2; demethylase
• UniProt Protein Name: Methyl-CpG-binding domain protein 2
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD2
• UniProt Synonym Gene Names: DMTase
• UniProt Entry Name: MBD2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

MBD2: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. May enhance the activation of some unmethylated cAMP-responsive promoters. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: nucleoplasm; heterochromatin; protein complex; cytoplasm; nuclear chromatin; histone deacetylase complex; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; nucleosomal DNA binding; siRNA binding; methyl-CpG binding; satellite DNA binding

Biological Process: cellular protein complex assembly; transcription, DNA-dependent; maternal behavior; negative regulation of gene expression, epigenetic; ATP-dependent chromatin remodeling; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; regulation of gene expression, epigenetic; regulation of cell proliferation