Human GMP synthase ELISA Kit | GMPS elisa kit

Human GMP synthase (GMPS) ELISA Kit

Cat. Num. AAA282332

• Gene Names: GMPS; GATD7
• Reactivity: Human
• Synonyms: GMP synthase; Human GMP synthase (GMPS) ELISA Kit; GMPS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GMPS. No significant cross-reactivity or interference between Human GMPS and analogues was observed.
• Sequence Length: 540

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GMPS elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GMPS in samples. An antibody specific for GMPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGMPS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GMPS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GMPS bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4504035
• NCBI GeneID: 8833
• NCBI Accession #: NP_003866.1
• NCBI GenBank Nucleotide #: NM_003875.2
• UniProt Accession #: P49915; A8K639; B4DXV7; F8W720
• Molecular Weight: 65,929 Da
• NCBI Official Full Name: GMP synthase
• NCBI Official Synonym Full Names: guanine monophosphate synthase
• NCBI Official Symbol: GMPS
• NCBI Official Synonym Symbols: GATD7
• NCBI Protein Information: GMP synthase [glutamine-hydrolyzing]
• UniProt Protein Name: GMP synthase [glutamine-hydrolyzing]
• Protein Family: GMP synthase
• UniProt Gene Name: GMPS

NCBI Description

In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]

Uniprot Description

GMPS: Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division. A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.

Protein type: EC 6.3.5.2; Ligase; Nucleotide Metabolism - purine; Oncoprotein; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 3q25.31

Cellular Component: cytosol

Molecular Function: ATP binding; GMP synthase (glutamine-hydrolyzing) activity; GMP synthase activity; pyrophosphatase activity

Biological Process: glutamine metabolic process; GMP biosynthetic process; purine nucleobase biosynthetic process; purine ribonucleoside monophosphate biosynthetic process

Disease: Leukemia, Acute Myeloid