GMPR recombinant protein

GMPR Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121891

• Gene Names: GMPR; GMPR1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: GMPR; GMPR Protein; Human; Recombinant (His Tag); Human GMPR/GMPR1 Protein (His Tag); GMPR1 Protein; guanosine monophosphate reductase; GMPR recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 150mM NaCl, 40% Glycerol, 1mM DTT, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser345

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human GMPR (NP_006868.3)(Met1-Ser345) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GMPR recombinant protein

Background: GMPR, also known as GMPR1, belongs to the IMPDH/GMPR family.This familyofenzymesincludesIMP dehydrogenaseandGMP reductase. These enzymes are involved inpurine metabolism and adopt aTIM barrelstructure. GMPR is an enzyme that catalyzes the irreversible and NADPH-dependent reductive deamination of GMP to IMP. GMPR functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.

References

Deng Y., et al.,(2002), NADPH-dependent GMP reductase isoenzyme of human (GMPR2). Expression, purification, and kinetic properties. Int. J. Biochem. Cell Biol. 34:1035-1050.Zhang J., et al., (2003), Cloning and functional characterization of GMPR2, a novel human guanosine monophosphate reductase, which promotes the monocytic differentiation of HL-60 leukemia cells.J. Cancer Res. Clin. Oncol. 129:76-83.Ivens A.C., et al.,(2005), The genome of the kinetoplastid parasite, Leishmania major.Science 309:436-442.

NCBI and Uniprot Product Information

• NCBI GI #: 156104880
• NCBI GeneID: 2766
• NCBI Accession #: NP_006868.3
• NCBI GenBank Nucleotide #: NM_006877.3
• UniProt Accession #: P36959; Q96HQ6
• Molecular Weight: 37,419 Da
• NCBI Official Full Name: GMP reductase 1
• NCBI Official Synonym Full Names: guanosine monophosphate reductase
• NCBI Official Symbol: GMPR
• NCBI Official Synonym Symbols: GMPR1
• NCBI Protein Information: GMP reductase 1; guanine monophosphate reductase; guanosine 5'-monophosphate oxidoreductase 1; guanosine monophosphate reductase 1
• UniProt Protein Name: GMP reductase 1
• Protein Family: GMP reductase
• UniProt Gene Name: GMPR
• UniProt Synonym Gene Names: GMPR1; Guanosine monophosphate reductase 1
• UniProt Entry Name: GMPR1_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the irreversible and NADPH-dependent reductive deamination of GMP to IMP. The protein also functions in the re-utilization of free intracellular bases and purine nucleosides.[provided by RefSeq, Oct 2009]

Uniprot Description

GMPR: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides. Belongs to the IMPDH/GMPR family.

Protein type: Nucleotide Metabolism - purine; Oxidoreductase; EC 1.7.1.7

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: cytosol

Molecular Function: GMP reductase activity; metal ion binding

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleotide metabolic process; response to cold; purine base metabolic process; purine salvage