Typical Testing Data/Standard Curve (for reference only)
Human Anti Ganglioside M1 Antibody ELISA Kit | GM1 elisa kit
Human Anti Ganglioside M1 Antibody ELISA Kit
Reactivity
Human
Synonyms
Anti Ganglioside M1 Antibody; Human Anti Ganglioside M1 Antibody ELISA Kit; GM1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of GM1-Ab. No significant cross-reactivity or interference between GM1-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GM1-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GM1 elisa kit
Intended Uses: This GM1-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GM1-Ab. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: GM1-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a Ganglioside M1 antigen and an GM1-Ab-HRP conjugate. The assay sample and buffer are incubated together with GM1-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GM1-Ab concentration since GM1-Ab from samples and GM1-Ab-HRP conjugate compete for the Ganglioside M1 antigen binding site. Since the number of sites is limited, as more sites are occupied by GM1-Ab from the sample, fewer sites are left to bind GM1-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM1-Ab concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GM1-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a Ganglioside M1 antigen and an GM1-Ab-HRP conjugate. The assay sample and buffer are incubated together with GM1-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GM1-Ab concentration since GM1-Ab from samples and GM1-Ab-HRP conjugate compete for the Ganglioside M1 antigen binding site. Since the number of sites is limited, as more sites are occupied by GM1-Ab from the sample, fewer sites are left to bind GM1-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM1-Ab concentration in each sample is interpolated from this standard curve.
Product Categories/Family for GM1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
21,410 Da
NCBI Official Full Name
Gamma-crystallin M1
UniProt Protein Name
Gamma-crystallin M1
UniProt Gene Name
GM1
UniProt Synonym Gene Names
Gamma-M1
UniProt Entry Name
CRGM1_CHIID
Uniprot Description
Crystallins are the dominant structural components of the vertebrate eye lens.
Similar Products
Product Notes
The Human GM1 gm1 (Catalog #AAA729305) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA729305 ELISA Kit recognizes Human GM1. It is sometimes possible for the material contained within the vial of "Anti Ganglioside M1 Antibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
