Glucose Regulated Protein 78 Recombinant Protein
Glucose Regulated Protein 78, Recombinant, Human (Grp78, BiP, KDEL)
Applications
ELISA, Western Blot
Purity
Highly Purified90% pure as determined by SDS-PAGE analysis.
Synonyms
Glucose Regulated Protein 78; Recombinant; Human (Grp78; BiP; KDEL); Glucose Regulated Protein 78 recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
90% pure as determined by SDS-PAGE analysis.
90% pure as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 1mM EDTA.
Applicable Applications for Glucose Regulated Protein 78 recombinant protein
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ATPase assays, as a Western Blot control, in Binding Assays and as an ELISA reference standard.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Glucose Regulated Protein 78 recombinant protein
GRP78 is a ubiquitously expressed, 78kD glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
Product Categories/Family for Glucose Regulated Protein 78 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
glucose regulated protein 78
Uniprot Description
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.
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Product Notes
The Glucose Regulated Protein 78 (Catalog #AAA637101) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glucose Regulated Protein 78 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ATPase assays, as a Western Blot control, in Binding Assays and as an ELISA reference standard. Researchers should empirically determine the suitability of the Glucose Regulated Protein 78 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucose Regulated Protein 78, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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