Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptojanin-1 (SYNJ1) Standard Curve.)
Mouse Synaptojanin-1 (SYNJ1) ELISA Kit | SYNJ1 elisa kit
Mouse Synaptojanin-1 (SYNJ1) ELISA Kit
Gene Names
Synj1; AA675315; mKIAA0910; A930006D20Rik
Reactivity
Mouse
Synonyms
Synaptojanin-1 (SYNJ1); Mouse Synaptojanin-1 (SYNJ1) ELISA Kit; SYNJ1; INPP5G; inositol 5-phosphatase (synaptojanin 1); synaptojanin-1; polyphosphoinositide phosphatase; SYNJ1 elisa kit
Reactivity
Mouse
Specificity
Mouse Synaptojanin-1 (SYNJ1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYNJ1. No significant cross-reactivity or interference between Mouse SYNJ1 and analogues was observed.
Sequence Length
1574
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptojanin-1 (SYNJ1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptojanin-1 (SYNJ1) Standard Curve.)
Related Product Information for SYNJ1 elisa kit
Description: This Mouse Synaptojanin-1 (SYNJ1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNJ1 in samples. An antibody specific for SYNJ1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYNJ1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNJ1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNJ1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Synaptojanin-1 is a phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2) that has a role in clathrin-coated pit dynamics. The authors found that synaptojanin-170, an alternatively spliced isoform of SYNJ1, binds EPS15, a clathrin coat-associated protein. Binding was mediated by the C-terminal region of synaptojanin-170, which contains 3 asparagine-proline-phenylalanine (NPF) repeats. This motif had been found to be the core of the binding site for the EH domains of EPS15. These findings suggested that SYNJ1 can be recruited to clathrin-coated pits via a multiplicity of interactions.These Synj1-deficient mice exhibited neurologic defects and died shortly after birth.
Background: Synaptojanin-1 is a phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2) that has a role in clathrin-coated pit dynamics. The authors found that synaptojanin-170, an alternatively spliced isoform of SYNJ1, binds EPS15, a clathrin coat-associated protein. Binding was mediated by the C-terminal region of synaptojanin-170, which contains 3 asparagine-proline-phenylalanine (NPF) repeats. This motif had been found to be the core of the binding site for the EH domains of EPS15. These findings suggested that SYNJ1 can be recruited to clathrin-coated pits via a multiplicity of interactions.These Synj1-deficient mice exhibited neurologic defects and died shortly after birth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Synaptojanin-1
NCBI Official Synonym Full Names
synaptojanin 1
NCBI Official Symbol
Synj1
NCBI Official Synonym Symbols
AA675315; mKIAA0910; A930006D20Rik
NCBI Protein Information
synaptojanin-1
UniProt Protein Name
Synaptojanin-1
Protein Family
UniProt Gene Name
Synj1
UniProt Synonym Gene Names
Kiaa0910
UniProt Entry Name
SYNJ1_MOUSE
Research Articles on SYNJ1
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Product Notes
The Mouse SYNJ1 synj1 (Catalog #AAA9716901) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716901 ELISA Kit recognizes Mouse SYNJ1. It is sometimes possible for the material contained within the vial of "Synaptojanin-1 (SYNJ1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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