Canine Glutamate ELISA Kit | Glu elisa kit

Canine Glutamate (Glu) ELISA Kit

Cat. Num. AAA2604553

• Gene Names: Gria1; GluA1; gluR-A
• Reactivity: Canine
• Synonyms: Glutamate; Canine Glutamate (Glu) ELISA Kit; Glu elisa kit

• Reactivity: Canine
• Sequence Length: 907

• Samples: Canine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 nmol/mL-0.312 nmol/mL
• Sensitivity: Up to 0.06 nmol/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Glu elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Canine Glu monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 29789269
• NCBI GeneID: 50592
• NCBI Accession #: NP_113796.1
• NCBI GenBank Nucleotide #: NM_031608.1
• Molecular Weight: 101,555 Da
• NCBI Official Full Name: glutamate receptor 1
• NCBI Official Synonym Full Names: glutamate ionotropic receptor AMPA type subunit 1
• NCBI Official Symbol: Gria1
• NCBI Official Synonym Symbols: GluA1; gluR-A
• NCBI Protein Information: glutamate receptor 1
• UniProt Protein Name: Glutamate receptor 1
• Protein Family: Glutamate mutase
• UniProt Gene Name: Gria1
• UniProt Synonym Gene Names: Glur1; GluR-1; GluA1
• UniProt Entry Name: GRIA1_RAT

NCBI Description

mouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning [RGD, Feb 2006]

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Membrane protein, multi-pass; Channel, calcium; Channel, ligand-gated; Membrane protein, integral

Cellular Component: asymmetric synapse; cell junction; cell soma; cell surface; cytosol; dendrite; dendritic shaft; dendritic spine; early endosome; endoplasmic reticulum; endoplasmic reticulum membrane; excitatory synapse; integral to membrane; ionotropic glutamate receptor complex; membrane; neuromuscular junction; neuron projection; plasma membrane; postsynaptic density; postsynaptic membrane; protein complex; recycling endosome; synapse; synaptic vesicle

Molecular Function: adenylate cyclase binding; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; beta-2 adrenergic receptor binding; extracellular-glutamate-gated ion channel activity; G-protein alpha-subunit binding; G-protein beta-subunit binding; identical protein binding; ionotropic glutamate receptor activity; myosin V binding; PDZ domain binding; protein binding; protein domain specific binding; protein homodimerization activity; protein kinase A binding; protein kinase binding; small GTPase binding

Biological Process: generation of action potential; ionotropic glutamate receptor signaling pathway; long-term memory; positive regulation of membrane potential; positive regulation of synaptic transmission; receptor internalization; regulation of receptor recycling; regulation of synaptic plasticity; regulation of synaptic transmission; response to arsenic; response to cocaine; response to drug; response to electrical stimulus; response to estradiol stimulus; response to lithium ion; response to organic cyclic substance; response to peptide hormone stimulus; response to toxin; spinal cord development; synaptic transmission; synaptic transmission, glutamatergic