Glutamate Dehydrogenase Enzyme | GLDH enzyme
Glutamate Dehydrogenase (Yeast) (GLDH)
Glucose-6-phosphate dehydrogenase < 0.1 %
Phosphogluconate dehydrogenase < 0.5 %
Glutamate dehydrogenase (NAD+) < 0.1 %
Glutathione reductase < 0.1 %
NADPH oxidase < 0.01 %
(for reduction of alpha-ketoglutarate to L-glutamate at 25 degree C and pH 7.6)
One mg of powder contains the following
Protein 700 +/- 200 ug
K-PO4 300 +/- 300 ug
NCBI and Uniprot Product Information
NCBI Description
This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.
Uniprot Description
Function: May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate
Catalytic activity: L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Enzyme regulation: Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme.
Subunit structure: Homohexamer.
Subcellular location: Mitochondrion matrix.
Involvement in disease: Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [
MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [
MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver. Ref.22 Ref.23 Ref.24 Ref.25
Sequence similarities: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
Research Articles on GLDH
Similar Products
Product Notes
The GLDH glud1 (Catalog #AAA682117) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glutamate Dehydrogenase, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
