Corticotropin Releasing Hormone (CRH) Recombinant Protein | CRH recombinant protein

Recombinant Corticotropin Releasing Hormone (CRH)

Cat. Num. AAA2010139

• Gene Names: Crh; CRF
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Corticotropin Releasing Hormone (CRH); Recombinant Corticotropin Releasing Hormone (CRH); CRH recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-QP LNFLQPEQPQ QPQPILIRMG EEYFLRLGNL NRSPAARLSP NSTPLTAGRG SRPSHDQAAA NFFRVLLQQL QMPQRPLDSS TELAERGAED ALGGHQGALE RERRSEEPPI SLDLTFHLLR EVLEMARAEQ LAQQAHSNRK LMEIIGK
• Sequence Length: 187

• Applicable Applications for CRH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gln39~Lys187 (Accession # P01143) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13591920
• NCBI GeneID: 81648
• NCBI Accession #: NP_112281.1
• NCBI GenBank Nucleotide #: NM_031019.1
• UniProt Accession #: P01143
• Molecular Weight: 18.3kDa
• NCBI Official Full Name: corticoliberin
• NCBI Official Synonym Full Names: corticotropin releasing hormone
• NCBI Official Symbol: Crh
• NCBI Official Synonym Symbols: CRF
• NCBI Protein Information: corticoliberin; corticotropin-releasing factor; corticotropin-releasing hormone
• UniProt Protein Name: Corticoliberin
• Protein Family: Corticoliberin
• UniProt Gene Name: Crh
• UniProt Synonym Gene Names: CRF
• UniProt Entry Name: CRF_RAT

NCBI Description

binds CRH receptors and mediates neuroendocrine stress response; may play a role in the consolidation of long term memory [RGD, Feb 2006]

Uniprot Description

Function: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Ref.3

Subunit structure: Interacts (via C-terminus) with CRFR1 (via N-terminal extracellular domain)

By similarity.

Subcellular location: Secreted Ref.3.

Sequence similarities: Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.