Mouse PDK4 Monoclonal Antibody | anti-PDK4 antibody

PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4, FLJ40832) (FITC)

Cat. Num. AAA6176441

• Applications: ELISA
• Purity: Purified
• Synonyms: PDK4; Monoclonal Antibody; PDK4 (Pyruvate Dehydrogenase Kinase; Isozyme 4; FLJ40832) (FITC); Pyruvate Dehydrogenase Kinase; FLJ40832; anti-PDK4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1A10
• Specificity: Recognizes PDK4.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 411

• Applicable Applications for anti-PDK4 antibody: ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: PDK4 (AAH40239.1, 1aa-411aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged PDK4 is approximately 0.1ng/ml as a capture antibody.)

Related Product Information for anti-PDK4 antibody

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq]

Product Categories/Family for anti-PDK4 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Alteration of energy metabolism in the pathogenesis of bile duct lesions in primary biliary cirrhosis. Harada K, Kakuda Y, Sato Y, Ikeda H, Shimoda S, Yamamoto Y, Inoue H, Ohta H, Kasashima S, Kawashima A, Nakanuma YJ Clin Pathol. 2013 Nov 29. doi: 10.1136/jclinpath-2013-201815. 2. The Increase in Cardiac Pyruvate Dehydrogenase Kinase-4 after Short-Term Dexamethasone Is Controlled by an Akt-p38-Forkhead Box Other Factor-1 Signaling Axis. Puthanveetil P, Wang Y, Wang F, Kim MS, Abrahani A, Rodrigues B.Endocrinology. 2010 May;151(5):2306-18. Epub 2010 Feb 24.

NCBI and Uniprot Product Information

• NCBI GI #: 25955471
• NCBI GeneID: 5166
• NCBI Official Full Name: PDK4 protein
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase 4
• NCBI Official Symbol: PDK4
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 4

NCBI Description

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]