Human Cytochrome P450 24A1 (CYP24A1) ELISA Kit | CYP24A1 elisa kit

Human Cytochrome P450 24A1 (CYP24A1) ELISA Kit

Cat. Num. AAA452136

• Gene Names: CYP24A1; CP24; HCAI; CYP24; HCINF1; P450-CC24
• Reactivity: Human
• Synonyms: Cytochrome P450 24A1 (CYP24A1); Human Cytochrome P450 24A1 (CYP24A1) ELISA Kit; CP24; CYP24; P450-CC24; Vitamin D 24-Hydroxylase; 1; 25-dihydroxyvitamin D(3) 24-hydroxylase; mitochondrial; Cytochrome P450 Family 24 Subfamily A Polypeptide 1; CYP24A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYP24A1. No significant cross-reactivity or interference between CYP24A1 and analogues was observed.
• Sequence Length: 514

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYP24A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYP24A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP24A1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CYP24A1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP24A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CYP24A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP24A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CYP24A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55770850
• NCBI GeneID: 1591
• NCBI Accession #: NP_000773.2
• NCBI GenBank Nucleotide #: NM_000782.4
• UniProt Accession #: Q07973; Q15807; Q32ML3; Q5I2W7
• Molecular Weight: 43,051 Da
• NCBI Official Full Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: cytochrome P450 family 24 subfamily A member 1
• NCBI Official Symbol: CYP24A1
• NCBI Official Synonym Symbols: CP24; HCAI; CYP24; HCINF1; P450-CC24
• NCBI Protein Information: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• UniProt Protein Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• Protein Family: 1,25-dihydroxyvitamin D(3) 24-hydroxylase
• UniProt Gene Name: CYP24A1
• UniProt Synonym Gene Names: CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.