General Hexokinase Assay Kit

Hexokinase Microplate Assay Kit

Cat. Num. AAA8243241

• Reactivity: General
• Applications: Functional Assay
• Synonyms: Hexokinase; Hexokinase Microplate Assay Kit; EC 2.7.1.1; Hexokinase type I; Hexokinase type II; Hexokinase type III; Hexokinase type IV (glucokinase); Hexokinase assay kit

• Reactivity: General

• Applicable Applications for Hexokinase assay kit: Functional Assay
• Samples: Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples
• Detection Range: 4 umol/L - 400 umol/L
• Preparation and Storage: Shipped and store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hexokinase assay kit

Background/Introduction: Hexokinases, which catalyze the ATP-dependent phosphorylation of aldo- and keto-hexoses to hexose-6-phosphate, catalyze the first step in a number of important biochemical pathways. In mammalian tissues, glucose is the predominant substrate for the hexokinases. There are four isozymes of hexokinase in mammalian tissue, (HK-I, -II, -III, and -IV), which differ in their kinetic and regulatory properties as well as tissue distribution and cofactor use. The hexokinases catalyze the first step in most of the relevant glucose metabolism pathways. Alterations in hexokinase activity are associated with multiple disorders such as X-linked muscular dystrophy, hemolytic anermias, and cancer. The Hexokinase Microplate Assay Kit provides a simple and direct procedure for measuring hexokinase activity in a variety of samples. Hexokinase activity is determined by a coupled enzyme assay, in which glucose is converted to glucose-6-phosphate by hexokinase, which is oxidized by glucose-6-phosphate dehydrogenase to form NADPH. The resulting NADPH reduces a colorless probe resulting in a colorimetric (340 nm) product proportional to the hexokinase activity present.