General Cysteine Assay Kit

Cysteine Microplate Assay Kit

Cat. Num. AAA8309602

• Reactivity: General
• Applications: Functional Assay
• Synonyms: Cysteine; Cysteine Microplate Assay Kit; (R)-2-Amino-3-mercaptopropanoic acid; (R)-Cysteine; 2-Amino-3-mercaptopropionic acid; Cystein; Half-cystine; L-(+)-Cysteine; L-Alanine 3-mercapto-; L-cisteina; L-Cys; L-Cystein; L-Cysteine; Thioserine; alpha-Amino-beta-thiolpropionic acid; beta-Mercaptoalanine; Cysteine assay kit

• Reactivity: General

• Applicable Applications for Cysteine assay kit: Functional Assay (FA)
• Samples: Tissue extracts, cell lysate, cell culture media and other biological fluids samples
• Detection Range: 20 mmol/L - 2000 mmol/L
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cysteine assay kit

Background/Introduction: Cysteine (CYS) is a sulfhydryl-containing amino acid and an important structural and functional part of proteins. In animals, cysteine is synthesized from transsulfuration of homocysteine, which is itself derived from metabolism of the amino acid methionine. Cystathionine beta-synthase catalyzes condensation of homocysteine with serine to form cystathionine, which is deaminated and hydrolyzed by Cystathionine beta-lyase to form cysteine and alpha-ketobutyrate. Because of its nucleophilic nature, the thiol group of cysteine has numerous biological functions. The formation of disulfide linkages between the thiol groups of cysteine residues helps to stabilize the tertiary and quaternarystructure of proteins. Cysteine, homocysteine (HCY), and other aminothiols exist in plasma in reduced, oxidized, and protein-bound forms, interacting with each other through redox pathways. Cysteine is the limiting precursor of the major intracellular antioxidant glutathione. The individuals with lower cysteine levels are more prone to damage from reactive oxygen species, which are generally removed either by thiols or by glutathione-linked enzymes. An elevated level of total cysteine also predicts adverse outcomes such as cardiovascular diseases and metabolic syndromes. Cysteine Microplate Assay Kit is a sensitive assay for determining Cysteine in various samples. Cysteine concentration is determined by sodium tungstate dihydrate. The reaction products can be measured at a colorimetric readout at 600 nm.