Typical Testing Data/Standard Curve (for reference only)
General Glycogen Branching Enzyme Assay Kit
Glycogen Branching Enzyme Microplate Assay Kit
Reactivity
General
Applications
Functional Assay
Synonyms
Glycogen Branching Enzyme; Glycogen Branching Enzyme Microplate Assay Kit; Glycogen Branching Enzyme assay kit
Reactivity
General
Sequence Length
766
Applicable Applications for Glycogen Branching Enzyme assay kit
Functional Assay (FA)
Samples
Tissue extracts, cell lysate, cell culture media and other biological fluids samples
Detection Range
0.01 mmol/L - 2 mmol/L
Preparation and Storage
Store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Glycogen Branching Enzyme assay kit
Background/Introduction: Glycogen branching enzyme (EC 2.4.1.18) is an enzyme that adds branches to the growing glycogen molecule during the synthesis of glycogen, a storage form of glucose. More specifically, during glycogen synthesis, a glucose 1-phosphate molecule reacts with uridine triphosphate (UTP) to become UDP-glucose, an activated form of glucose. The activated glucosyl unit of UDP-glucose is then transferred to the hydroxyl group at the C-4 of a terminal residue of glycogen to form an alpha-1,4-glycosidic linkage, a reaction catalyzed by glycogen synthase. Importantly, glycogen synthase can only catalyze the synthesis of alpha-1,4-glycosidic linkages. Since glycogen is a readily mobilized storage form of glucose, the extended glycogen polymer is branched by glycogen branching enzyme to provide glycogen breakdown enzymes, such as glycogen phosphorylase, with a large number of terminal residues for rapid degradation. Branching also importantly increases the solubility and decreases the osmotic strength of glycogen. Glycogen branching enzyme Microplate Assay Kit is a sensitive assay for determining Glycogen branching enzyme in various samples. Glycogen branching enzyme activity is determined by iodine. The reaction products can be measured at a colorimetric readout at 660 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
glycogen branching enzyme
Similar Products
Product Notes
The General Glycogen Branching Enzyme (Catalog #AAA8309601) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8309601 Assay Kit recognizes General Glycogen Branching Enzyme. AAA Biotech's Glycogen Branching Enzyme can be used in a range of immunoassay formats including, but not limited to, Functional Assay (FA). Researchers should empirically determine the suitability of the Glycogen Branching Enzyme for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycogen Branching Enzyme, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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