Western Blot (WB)
(Western blot analysis of anti-NMD3 Pab in mouse heart (left) and T47D (right)tissue lysates (35ug/lane). NMD3(arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse NMD3 Polyclonal Antibody | anti-NMD3 antibody
NMD3 Antibody (C-term)
Gene Names
NMD3; CGI-07
Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NMD3; Polyclonal Antibody; NMD3 Antibody (C-term); 60S ribosomal export protein NMD3; hNMD3; anti-NMD3 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NMD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-440 amino acids from the C-terminal region of human NMD3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
410-440
Sequence Length
503
Applicable Applications for anti-NMD3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of anti-NMD3 Pab in mouse heart (left) and T47D (right)tissue lysates (35ug/lane). NMD3(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-NMD3 Pab in mouse heart (left) and T47D (right)tissue lysates (35ug/lane). NMD3(arrow) was detected using the purified Pab.)
Related Product Information for anti-NMD3 antibody
It has been suggested that NMD3 is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. The NMD3 protein sequence does not contain readily recognizable motifs of known function. However, NMD3 orthologues display an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization.
Product Categories/Family for anti-NMD3 antibody
References
Trotta, C.R., et al., EMBO J. 22(11):2841-2851 (2003).
Ho, J.H. et al., Mol. Cell. Bio., 19(3):2389-2399 (1999).
Ho, J.H. et al., Mol. Cell. Bio., 19(3):2389-2399 (1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57603
NCBI Official Full Name
60S ribosomal export protein NMD3
NCBI Official Synonym Full Names
NMD3 ribosome export adaptor
NCBI Official Symbol
NMD3
NCBI Official Synonym Symbols
CGI-07
NCBI Protein Information
60S ribosomal export protein NMD3
UniProt Protein Name
60S ribosomal export protein NMD3
Protein Family
UniProt Gene Name
NMD3
UniProt Synonym Gene Names
hNMD3
UniProt Entry Name
NMD3_HUMAN
NCBI Description
Ribosomes are composed of 60S and 40S subunits that are assembled in the nucleolus and exported to the cytoplasm through nuclear pore complexes in the nuclear envelope. NMD3 is an adaptor for 60S subunit export via the CRM1 (XPO1; MIM 602559) pathway (Trotta et al., 2003 [PubMed 12773398]).[supplied by OMIM, Mar 2008]
Uniprot Description
NMD3: Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit. Belongs to the NMD3 family.
Protein type: Nucleolus; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 3q26.1
Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding, bridging; ribosomal large subunit binding
Biological Process: protein transport; ribosomal large subunit export from nucleus
Research Articles on NMD3
Similar Products
Product Notes
The NMD3 nmd3 (Catalog #AAA9212271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 410-440. The NMD3 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NMD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the NMD3 nmd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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