Mouse Growth Differentiation Factor 2 ELISA Kit | GDF2 elisa kit

Mouse Growth Differentiation Factor 2 ELISA Kit

Cat. Num. AAA21681

• Gene Names: GDF2; BMP9; BMP-9
• Reactivity: Mouse
• Synonyms: Growth Differentiation Factor 2; Mouse Growth Differentiation Factor 2 ELISA Kit; GDF2 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse GDF2 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 6.25 pg/ml - 200 pg/ml.
• Sensitivity: 1.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GDF2 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse GDF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GDF2 and GDF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GDF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GDF2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GDF2 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse GDF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GDF2 and GDF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GDF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7705308
• NCBI GeneID: 2658
• NCBI Accession #: NP_057288.1
• NCBI GenBank Nucleotide #: NM_016204.1
• UniProt Accession #: Q9UK05; Q5VSQ9; Q9Y571
• Molecular Weight: 47,320 Da
• NCBI Official Full Name: growth/differentiation factor 2
• NCBI Official Synonym Full Names: growth differentiation factor 2
• NCBI Official Symbol: GDF2
• NCBI Official Synonym Symbols: BMP9; BMP-9
• NCBI Protein Information: growth/differentiation factor 2; GDF-2; bone morphogenetic protein 9
• UniProt Protein Name: Growth/differentiation factor 2
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF2
• UniProt Synonym Gene Names: BMP9; GDF-2; BMP-9
• UniProt Entry Name: GDF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF2: Could be involved in bone formation. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; positive regulation of interleukin-8 production; regulation of apoptosis; BMP signaling pathway; positive regulation of endothelial cell differentiation; angiogenesis; vasculogenesis; positive regulation of BMP signaling pathway; ossification; negative regulation of blood vessel endothelial cell migration; osteoblast differentiation; patterning of blood vessels; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; negative regulation of angiogenesis; negative regulation of DNA replication; regulation of MAPKKK cascade; cartilage development; negative regulation of endothelial cell proliferation; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; cell development; growth

Disease: Telangiectasia, Hereditary Hemorrhagic, Type 5