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Typical Testing Data/Standard Curve (for reference only)

Human growth differentiation factor 2 ELISA Kit | GDF2 elisa kit

human growth differentiation factor 2, GDF2 ELISA Kit

Gene Names
GDF2; BMP9; HHT5; BMP-9
Reactivity
Human
Synonyms
growth differentiation factor 2; human growth differentiation factor 2; GDF2 ELISA Kit; BMP-9; BMP9; bone morphogenetic protein 9; GDF2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human GDF2. No significant cross-reactivity or interference between human GDF2 and analogues was observed.
Sequence Length
429
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
1.56 pg/ml -10 0 pg/ml.
Sensitivity
The minimum detectable dose of human GDF2 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GDF2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GDF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GDF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,320 Da
NCBI Official Full Name
growth/differentiation factor 2 preproprotein
NCBI Official Synonym Full Names
growth differentiation factor 2
NCBI Official Symbol
GDF2
NCBI Official Synonym Symbols
BMP9; HHT5; BMP-9
NCBI Protein Information
growth/differentiation factor 2; GDF-2; bone morphogenetic protein 9
UniProt Protein Name
Growth/differentiation factor 2
UniProt Gene Name
GDF2
UniProt Synonym Gene Names
BMP9; GDF-2; BMP-9
UniProt Entry Name
GDF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. [provided by RefSeq, Jan 2014]

Uniprot Description

Function: Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.3 PublicationsManual assertion based on experiment in:Ref.6

Research Articles on GDF2

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Product Notes

The Human GDF2 gdf2 (Catalog #AAA703200) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703200 ELISA Kit recognizes Human GDF2. It is sometimes possible for the material contained within the vial of "growth differentiation factor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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