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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AGPAT5 protein (Right) or empty vector (Left) detected with AGPAT5 antibody)

Mouse AGPAT5 Monoclonal Antibody | anti-AGPAT5 antibody

AGPAT5 antibody

Gene Names
AGPAT5; LPAATE; 1AGPAT5
Applications
Immunohistochemistry, Western Blot
Purity
AGPAT5 antibody was purified by affinity chromatography.
Synonyms
AGPAT5; Monoclonal Antibody; AGPAT5 antibody; Monoclonal AGPAT5; Anti-AGPAT5; 1AGPAT5; LPAATE; Lysophosphatidic acid acyltransferase epsilon; AGPAT 5; AGPAT-5; anti-AGPAT5 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2C4
Purity/Purification
AGPAT5 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
356
Applicable Applications for anti-AGPAT5 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:150
WB: 1:2000
Biological Significance
This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis.
Cross-Reactivity
Human
Immunogen
AGPAT5 antibody was raised in Mouse using full length human recombinant protein of human AGPAT5 produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AGPAT5 protein (Right) or empty vector (Left) detected with AGPAT5 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AGPAT5 protein (Right) or empty vector (Left) detected with AGPAT5 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of AGPAT5 protein in paraffin embedded Human pancreas tissue using AGPAT5 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of AGPAT5 protein in paraffin embedded Human pancreas tissue using AGPAT5 antibody)
Related Product Information for anti-AGPAT5 antibody
Mouse monoclonal AGPAT5 antibody
Product Categories/Family for anti-AGPAT5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
42,072 Da
NCBI Official Full Name
AGPAT5 protein, partial
NCBI Official Synonym Full Names
1-acylglycerol-3-phosphate O-acyltransferase 5
NCBI Official Symbol
AGPAT5
NCBI Official Synonym Symbols
LPAATE; 1AGPAT5
NCBI Protein Information
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon
UniProt Protein Name
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon
UniProt Gene Name
AGPAT5
UniProt Synonym Gene Names
1-AGP acyltransferase 5; 1-AGPAT 5; LPAAT-epsilon
UniProt Entry Name
PLCE_HUMAN

NCBI Description

This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. A pseudogene of this gene is present on the Y chromosome. [provided by RefSeq, Aug 2014]

Uniprot Description

AGPAT5: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: EC 2.3.1.51; Membrane protein, integral; Transferase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; mitochondrion; integral to membrane; nuclear envelope

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; hemopoietic progenitor cell differentiation; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; phospholipid biosynthetic process

Research Articles on AGPAT5

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Product Notes

The AGPAT5 agpat5 (Catalog #AAA838475) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AGPAT5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the AGPAT5 agpat5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGPAT5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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