Rabbit anti-Human GCAT Polyclonal Antibody | anti-GCAT antibody

GCAT, NT (GCAT, KBL, 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, Aminoacetone synthase, Glycine acetyltransferase) (MaxLight 490)

Cat. Num. AAA6301539

• Gene Names: GCAT; KBL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: GCAT; Polyclonal Antibody; NT (GCAT; KBL; 2-amino-3-ketobutyrate coenzyme A ligase; mitochondrial; Aminoacetone synthase; Glycine acetyltransferase) (MaxLight 490); Glycine acetyltransferase; anti-GCAT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-GCAT antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the N-terminal region of human GCAT.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GCAT (arrow) using rabbit polyclonal GCAT Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene.)

Related Product Information for anti-GCAT antibody

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14.

Product Categories/Family for anti-GCAT antibody

Antibodies; Enzymes; Acetyltransferase

References

Jacquot, C., et al. Anticancer Res. 22(4):2229-2235(2002). Edgar, A.J., et al. Eur. J. Biochem. 267(6):1805-1812(2000). Tressel, T., et al. J. Biol. Chem. 261(35):16428-16437(1986).

NCBI and Uniprot Product Information

• NCBI GI #: 7657118
• NCBI GeneID: 23464
• NCBI Accession #: NP_055106.1
• NCBI GenBank Nucleotide #: NM_014291.3
• UniProt Accession #: O75600; Q6ZWF1; Q96CA9; E2QC23
• Molecular Weight: 47,974 Da
• NCBI Official Full Name: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: glycine C-acetyltransferase
• NCBI Official Symbol: GCAT
• NCBI Official Synonym Symbols: KBL
• NCBI Protein Information: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; AKB ligase; aminoacetone synthase; glycine acetyltransferase; 2-amino-3-ketobutyrate-CoA ligase
• UniProt Protein Name: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
• UniProt Gene Name: GCAT
• UniProt Synonym Gene Names: KBL; AKB ligase
• UniProt Entry Name: KBL_HUMAN

NCBI Description

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]

Uniprot Description

GCAT: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Mitochondrial; Transferase; EC 2.3.1.29

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; mitochondrion

Molecular Function: glycine C-acetyltransferase activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; biosynthetic process; threonine catabolic process to pyruvate